On the consistency of different methods for detecting aldosterone concentration in blood
10.3760/cma.j.issn.1000-6699.2019.11.003
- VernacularTitle: 不同方法检测血醛固酮浓度的一致性比较
- Author:
Fengfan ZHENG
1
;
Ying SONG
;
Shumin YANG
;
Jinbo HU
;
Wenwen HE
;
Zhihong WANG
;
Qingfeng CHENG
;
Qifu LI
Author Information
1. Department of Endocrinology, the First Affiliated Hospital of Chongqing Medical University, The Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
- Publication Type:Journal Article
- Keywords:
Aldosterone;
Liquid chromatography-tandem mass spectrometry;
Chemiluminescent assays;
Radioimmunoassay
- From:
Chinese Journal of Endocrinology and Metabolism
2019;35(11):934-938
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the consistency of different methods for detecting aldosterone concentration in blood and to establish a reference interval of serum aldosterone concentration by liquid chromatography-tandem mass spectrometry(LC-MS/MS).
Methods:Concentrations of blood aldosterone were measured by LC-MS/MS, chemiluminescent assays (Diasorin, Domestic A and B systems) and radioimmunoassay (RIA) in 138 healthy adults, 67 patients with essential hypertension and 23 patients with primary aldosteronism.
Results:Aldosterone concentrations measured by various methods were quiet different(P<0.01). Spearman correlation analysis showed that the correlation coefficient were 0.776(P<0.01) between CLIA (Diasorin) and LC-MS/MS, 0.805(P<0.01) between CLIA (Domestic A) and LC-MS/MS, 0.058(P>0.05) between CLIA(Domestic B) and LC-MS/MS, 0.338(P<0.01) as well as between RIA and LC-MS/MS. Bland-Altman analysis for the measurements showed poor consistency among methods for aldosterone concentrations. The reference interval was 15.2-222.2 pg/ml for serum aldosterone concentrations by LC-MS/MS.
Conclusions:There are significant differences of blood aldosterone concentrations determined by different methods. Clinically, a specified reference interval might be needed while using different methods.
