Expression of micro RNA-561 in esophageal squamous cell carcinoma and its clinical significance
10.3760/cma.j.issn.1673-4904.2019.12.012
- VernacularTitle: 食管鳞状细胞癌患者微小RNA-561的表达及其临床意义
- Author:
Zheng ZHAO
1
;
Yi LI
;
Feixiang YANG
Author Information
1. Department of Clinical Laboratory, Dongfeng Hospital Affiliated of Hubei University of Medicine, Hubei Shiyan 442000, China
- Publication Type:Journal Article
- Keywords:
Esophageal neoplasms;
MicroRNA-561;
Eca109;
Kyse150;
Het-1a
- From:
Chinese Journal of Postgraduates of Medicine
2019;42(12):1107-1111
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the clinical significance and expression of microRNA(miR)-561 in esophageal squamous cell carcinoma.
Methods:From January 2015 to February 2016, 96 specimens of adjacent tissues and cancer tissues from patients with esophageal squamous cell carcinoma who underwent surgery in Dongfeng Hospital Affiliated of Hubei University of Medicine were selected to detect the expression level of miR-561. Cell culture experiments were used to detect the expression level of miR-561 in Het-1a, Kyse150 and Eca109 cell lines. The correlation between clinical features and the expression level of miR-561 was counted. The expression level of miR-561 in patients with esophageal squamous cell carcinoma was analyzed. The relationship between the level of miR-561 and prognosis was investigated.
Results:Relative expression of miR-561 in esophageal squamous cell carcinoma cell lines Eca109 and Kyse150 (1.61 ± 0.30, 1.21 ± 0.28) was significantly lower than that in Het-1a (2.56 ± 0.51), and the difference was statistically significant (P < 0.05). The relative expression of miR-561 in esophageal squamous cell carcinoma tissues was significantly lower than that in adjacent tissues (0.80 ± 0.17 vs. 1.51 ± 0.42), and the difference was statistically significant (P < 0.05). The expression of miR-561 in esophageal squamous cell carcinoma was correlated with alcohol drinking history, lymph node metastasis, differentiation degree and tumor stage (P < 0.05). Cox multivariate regression analysis showed that the independent risk factors affecting the prognosis of patients were the expression of miR-561, TNM stage and lymph node metastasis (P < 0.05). The survival rate of patients with esophageal squamous cell carcinoma whose expression of miR-561 did not decrease at follow-up was significantly higher than that of patients with decreased expression of miR-561 (P < 0.05).
Conclusions:In patients with esophageal squamous cell carcinoma, the expression of miR-561 is low, and the development, occurrence and prognosis of esophageal squamous cell carcinoma are closely related with it.
