Genetic characteristics of five human adenovirus type 53 strains isolated from Taiyuan city in 2016, China
10.3760/cma.j.issn.0253-9624.2019.12.013
- VernacularTitle: 2016年太原市5株人腺病毒53型病毒分离株基因特征分析
- Author:
Jitao WANG
1
;
Xiaoling NING
2
;
Dequan MENG
1
;
Jiane GUO
1
;
Lifeng ZHAO
1
;
Naiying MAO
3
;
Hong LI
4
;
Wenbo XU
3
;
Zhen ZHU
3
Author Information
1. Department of Microbiology Test, Taiyuan Center for Disease Control and Prevention, Taiyuan 030001, China
2. Comprehensive Inspection Section, Shanxi Eye Hospital, Taiyuan 030002, China
3. NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 102206, China
4. Medicine College, Anhui University of Science and Technology, Huainan 232001, China
- Publication Type:Journal Article
- Keywords:
Keratoconjunctivitis;
Adenovirus, human;
Genetic characteristic
- From:
Chinese Journal of Preventive Medicine
2019;53(12):1271-1277
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The genetic characteristics of the human adenovirus type 53 (HAdV-53) strains isolated from Taiyuan city of Shanxi Province were studied to obtain the baseline data of their molecular characteristics.
Methods:Conjunctival swabs (n=79) were collected from epidemic keratoconjunctivitis (EKC) patients in Shanxi eye Hospital in 2016, and five HAdV-53 strains were obtained after virus isolation and identification based on the three major capsid genes sequences including Penton base, Hexon and Fiber gene. And the corresponding sequences of global epidemic HAdV-53 strains and the strains with the same genetic origin as HAdV-53 were also downloaded from GenBank database, and then the three gene database were established, respectively. With the database, phylogenetic tree was constructed, and the genetic and molecular evolutionary characteristics were analyzed with bioinformatics software.
Results:Five HAdV-53 strains in Shanxi Province in 2016 showed high consistency with the HAdV-53 strains prevalent in other countries in 1996-2014 (>99.8%). All HAdV-53 strains were in the same evolutionary branch with their recombinant source genotypes (HAdV-37 and HAdV-8) in Penton base and Fiber gene, respectively, and maintained a high degree of consistency in gene sequences. In Hexon gene, HAdV-53 strains were more closed to its recombinant source genotype HAdV-22, the nucleotide and amino acid sequences between two types were highly homologous, while HAdV-53 and HAdV-22 belonged to different evolutionary branches, and the evolution rate of HAdV-53 based on Hexon gene was 3.51×10-5 substitution/site/year.
Conclusion:HAdV-53 has become an important new ocular infectious pathogen of Taiyuan. HAdV-53 strain are relatively conservative and stable based on Penton base, Hexon, and Fiber gene.
