Characteristics and dynamics of HIV-1 subtype distribution among injected drug users in Guangzhou, 2008 - 2015
10.3760/cma.j.issn.0254-6450.2019.12.023
- VernacularTitle: 广州市2008-2015年注射吸毒人群HIV-1亚型分布特征及变化趋势
- Author:
Yuteng ZHAO
1
;
Zhigang HAN
2
;
Hao WU
1
;
Yali ZHANG
3
;
Fei ZHONG
1
;
Kai GAO
1
;
Huifang XU
1
Author Information
1. Department of AIDS Control and Prevention, Guangzhou Municipal Center for Disease Control and Prevention, Guangzhou 510440, China
2. Department of Operational Control, Guangzhou Municipal Center for Disease Control and Prevention, Guangzhou 510440, China
3. School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China
- Publication Type:Journal Article
- Keywords:
HIV-1;
Subtype;
Injected drug users;
Characteristics
- From:
Chinese Journal of Epidemiology
2019;40(12):1629-1633
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the characteristics and dynamics of individuals with HIV-1 subtype infection among injected drug users (HIV infection IDU) in Guangzhou between 2008 and 2015.
Methods:HIV-1 RNAs were extracted from serum samples of the individuals that were newly diagnosed with HIV-1 infection among IDUs living in Guangzhou, between 2008 and 2015. The Pol gene segments of HIV-1 genome from these RNA samples were amplified by nested reverse transcription polymerase chain reaction (Nested-PCR) and sequenced. Subsequently, phylogenetic tree was reconstructed using both pol sequences of samples and references before the subtype of HIV-1 was determined. Distributions of HIV-1 subtypes detected in IDUs with different demographic characteristics in different years were compared.
Results:A total of 437 pol gene segments were successfully obtained from 517 serum samples of HIV infection IDUs. The average age of 437 HIV infected IDUs was 37.37 years with standard deviation as 8.17 years. 51.5% (225/437) of the HIV infected IDU that registered residence were not in Guangdong. The Guangxi Registered residents were accounted for 54.2% (122/225). Proportion of subtype CRF07_BC (46.5%) appeared the highest, followed by CRF01_AE (24.3%), CRF08_BC (23.3%) and other subtypes (5.9%). The annual proportions of subtype CRF07_BC (trend χ2=19.703, P=0.006) and CRF08_BC (trend χ2=25.718, P=0.001) were significantly different. The proportion of subtype CRF07_BC decreased from 56.9% to 34.2% (trend χ2=15.139, P=0.000), while the proportion of CRF08_BC increased from 11.8% to 37.0% (trend χ2=22.577, P=0.000). The proportion of CRF08_BC was significantly higher in the HIV infected IDUs with Guangxi residence (Monte Carlo simulation of exact probability P=0.000, 99%CI: 0.000-0.000).
Conclusions:CRF07_BC, CRF01_ AE and CRF08_BC were the predominant HIV-1 subtypes while multiple subtypes were co-circulated among the HIV infected IDUs in Guangzhou, between 2008 and 2015. Behavioral intervention set for HIV infected IDUs with Guangxi residence should be strengthened in Guangzhou.
