Mechanism of Silencing HOXC8 Increases Radiosensitivity of A549 Cells
10.3760/cma.j.issn.1004-4221.2019.12.014
- VernacularTitle: 沉默HOXC8增加A549细胞对放射敏感性作用机制研究
- Author:
Rui SONG
1
;
Jinjin YUAN
;
Ge HOU
;
Jun YANG
;
Xiaojuan CHEN
;
Zongwen LIU
Author Information
1. Department of Radiation Oncology, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, China
- Publication Type:Journal Article
- Keywords:
HOXC8 gene;
A549 cell line;
TGF-β
1;
Radiosensitivity
- From:
Chinese Journal of Radiation Oncology
2019;28(12):942-944
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and potential mechanism of HOXC8 on the radiosensitivity of non-small cell lung cancer cell line A549, aiming to provide novel ideas for clinical combined treatment.
Methods:The A549 cells with stable knockdown of HOXC8 were constructed by using lentivirus and validated by qPCR and Western blot. The radiosensitivity of A549 stable cell line was assessed by plate clone formation assay. The expression levels of TGF-β1 and the proteins in the downstream signal pathway after knockdown of HOXC8 were detected by Western blot.
Results:The A549 cells with stable knockdown of HOXC8 were successfully constructed. The viability and clonogenic capacity of A549 cells were significantly reduced after silencing HOXC8. Silencing HOXC8 also increased the sensitivity of A549 cells to radiotherapy and significantly inhibited the expression of TGF-β1 and p-Smad2/3 proteins in the downstream signaling pathway.
Conclusion:Silencing HOXC8 can increase the sensitivity of A549 cells to radiotherapy probably by inhibiting TGF-β1 signaling transduction. HOXC8 might play an important role in A549 cells.
