Effect of lncRNA CRNDE targeting miR-384 on radiosensitivity of colorectal cancer cells
10.3760/cma.j.issn.0254-5098.2019.12.003
- VernacularTitle: LncRNA CRNDE靶向miR-384影响结直肠癌细胞放射敏感性的研究
- Author:
Xiantao SUN
1
;
Yanbang LIAN
2
;
Yang BAI
1
;
Chao YANG
1
;
Shengyun HU
1
;
Guixian WANG
1
Author Information
1. Department of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
2. Department of Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
- Publication Type:Journal Article
- Keywords:
Colorectal cancer;
Colorectal neoplasia differentially expressed(CRNDE);
miR-384;
Radiosensitivity
- From:
Chinese Journal of Radiological Medicine and Protection
2019;39(12):893-898
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of LncRNA CRNDE on radiosensitivity of colorectal cells and underlying mechanism.
Methods:Colorectal cancer HT-29 cells were transfected with CRNDE shRNA and the interference efficiency was determined by Real time PCR. HT-29 cells transfected with CRNDE shRNA or co-transfected with CRNDE shRNA and miR-384 inhibitor were irradiated at 8 Gy dose, then cell proliferation and apoptosis were detected by MTT and flow cytometry assay, respectively, and cell radiosensitivity was evaluated by cloning assay. It was predicted by a bioinformatics software that CRNDE and miR-384 have complementary binding sites, and this was identified by a luciferase reporting system.
Results:CRNDE shRNA reduced the expression of CRNDE in HT-29 cells(1.00±0.08 vs. 0.42±0.06, t=10.051, P<0.05). Both CRNDE shRNA and radiation inhibited the proliferation and induced apoptosis of HT-29 cells, and their combination treatment had synergistic effect in apoptosis induction [Apoptosis rates: (2.27±0.13)%, (23.58±2.35)%, (26.91±2.81)%, (36.84±3.24)%, F=24.660, P<0.05; A values: 0.45±0.06, 0.30±0.02, 0.28±0.03, 0.20±0.02, F=106.207, P<0.05]. Transfection of CRNDE shRNA increased the radiosensitivity of HT-29 cells with a radiosensitization ratio of 1.374. CRNDE negatively regulated the expression of its target miR-384. The miR-384 inhibitor antagonized the effect of CRNDE shRNA on proliferation inhibition and apoptosis promotion of radiation-treated colorectal cancer cells.
Conclusions:Down-regulation of LncRNA expression enhances the radiosensitivity of colorectal cancer cells by regulating miR-384 expression.
