High pressure increases the express of TNF-α and IL-1β in BV2 microglia
10.3760/cma.j.issn.1671-0282.2019.12.009
- VernacularTitle: 高压环境对BV2小胶质细胞TNF-α和IL-1β表达的影响
- Author:
Fan YANG
1
;
Xusheng LI
;
Ya LI
;
Xiaoyu LIU
;
Mengting LIU
;
Hongguang DING
;
Hongke ZENG
Author Information
1. Guangdong Provincial People's Hospital, Affiliated to South China University of Technology, Guangzhou 510080, China
- Publication Type:Journal Article
- Keywords:
Pressure;
Microglia;
TNF-α
;
IL-1β
- From:
Chinese Journal of Emergency Medicine
2019;28(12):1507-1510
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aimed to explore whether high pressure would increase expression of TNF-a and IL-1β.
Methods:BV2 microglia cells were treated with a self-made device. BV2 microglia cells were randomly divided into five groups according to different pressures: control group, 20 mmHg group, 25 mmHg group, 30 mmHg group, and 35 mmHg group. BV2 microglia cells were randomly divided into five groups according to different intervention time: control group, 6 h group, 12 h group, 24 h group. TNF-α and IL-1β expression were assessed by Western Blotting or double immunofluorescence.
Results:The 30 mmHg group had the highest expression levels of TNF-α and IL-1β as compared with control group (both P<0.01), 6 h group (both P<0.01)、12 h group (TNF-α: P<0.05; IL-1β: P< 0.01).30 mmHg group had the highest expression levels of TNF-α and IL-1β as compared with control group (bothP<0.01), 20 mmHg group (both P<0.01), and 25 mmHg group (TNF-α: P<0.05; IL-1β: P<0.01). The expression levels of TNF-α and L-1β were not different between 30 mmHg group and 35 mmHg group (both P>0.05).
Conclusions:High pressure may increase the expression levels of TNF-α and IL-1β of microglia.
