Preparation of monoclonal antibodies against pneumococcal polysaccharide and hepatitis B virus surface protein

Wen Qian; Ying Zhang; Nanping Chen; Yuqiu Chen; Lili Wang; Kai WU; Min Chen; Jing Shi; Qihan LI

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Preparation of monoclonal antibodies against pneumococcal polysaccharide and hepatitis B virus surface protein

VernacularTitle: 抗肺炎多糖和乙肝表面蛋白的单克隆抗体制备
Author: Wen QIAN ^{1
,

2} ; Ying ZHANG ¹ ; Nanping CHEN ³ ; Yuqiu CHEN ³ ; Lili WANG ³ ; Kai WU ³ ; Min CHEN ³ ; Jing SHI ³ ; Qihan LI ¹
Author Information

1. Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming 650118, China
2. Walvax Biotechnology Co. Ltd, Kunming 650106, China
3. Walvax Biotechnology Co. Ltd, Kunming 650106, China
Publication Type:Journal Article
Keywords: Conjugates; Monoclonal antibody; Pneumonia polysaccharide; Hybridoma cells; Hepatitis B virus surface protein
From:Chinese Journal of Microbiology and Immunology 2019;39(12):926-932
CountryChina
Language:Chinese
Abstract: Objective:To prepare monoclonal antibodies against pneumonia serotype 33F polysaccharides (Pn33Fps) and hepatitis B virus (HBV) surface proteins (HBs) by using the conjugate of Pn33Fps and HBs as antigen.
Methods:The conjugate of Pn33Fps and HBs was used as antigen to immunize mice with different immune doses, different immune procedures and different immune sites. Mouse spleen cells with higher antibody level after immunization were isolated and fused with SP2/0 myeloma cells. The hybridoma cells were screened specifically with Pn33Fps or HBs to prepare corresponding monoclonal antibodies.
Results:Serum antibodies against Pn33Fps and HBs were induced by immunizing mice with the conjugate. Monoclonal cell lines capable of continuously expressing antibodies against Pn33Fps or HBs were obtained. It has been proved that the recovery rates of samples of Pn33Fps and HBs prepared in three batches tested with ascites monoclonal antibodies prepared by these two monoclonal cell lines were between 95% and 105%.
Conclusions:Monoclonal antibodies against Pn33Fps and HBs could be prepared simultaneously by immunizing mice with the conjugate of Pn33Fps and HBs and used for the quantitative detection of Pn33Fps and HBs.