Distribution of cefuroxime in ocular tissues of rabbit after intravenous administration
10.3760/cma.j.issn.2095-0160.2019.12.006
- VernacularTitle: 头孢呋辛静脉注射后在兔眼内组织的分布
- Author:
Ruizhong GONG
1
;
Li WANG
1
;
Chen WANG
1
;
Yingxia ZHOU
1
;
Cuihong LI
1
;
Lin ZHANG
1
;
Zetao LIU
2
;
Liping YANG
2
Author Information
1. Department of Pharmacy, Shanxi Eye Hospital, Taiyuan 030002, China
2. Shanxi Provincial Institute of Food and Drug Control, Taiyuan 030000, China
- Publication Type:Journal Article
- Keywords:
Cefuroxime/pharmacokinetics;
Intraocular penetration;
High performance liquid chromatography
- From:
Chinese Journal of Experimental Ophthalmology
2019;37(12):967-970
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the distribution and pharmacokinetics of cefuroxime in rabbit eyes after intravenous administration.
Methods:Thirty-five rabbits were randomly divided into 7 groups by random number table method, with 5 rabbits in each group.The rabbits in blank control group were feed without any treatment, the rest rabbits were injected with 40.63 mg/kg cefuroxime intravenously.The rabbits were sacrificed at 0.5 hour, 1.0 hour, 1.5, 2.0, 2.5 and 3.0 hours after injection, and the eyeballs were immediately dissected.The concentration of drug in different ocular tissues was detected by high performance liquid chromatography, and the pharmacokinetic parameters in eyes were computed by the DAS software.This study was approved by the Experimental Animal Ethics Committee of Shanxi Provincial Eye Hospital (201802b).
Results:The peak concentrations (Cmax) of cefuroxime were (11.63±0.20), (1.59±0.05), (1.51±0.08), (0.99±0.07), (1.55±0.08) and (8.57±0.17)μg/ml in aqueous humor, iris-ciliary body, vitreous body, retinal-choroid, cornea and sclera, respectively.The times to peak (Tmax) were 1.5 hours, 1.0, 1.0, 0.5, 1.0 and 0.5 hour in aqueous humor, iris-ciliary body, vitreous body, retinal-choroid, cornea and sclera, respectively.The areas under drug time curve (AUC0-t) were (26.60±0.62), (6.22±0.84), (5.86±0.16), (3.75±0.45), (5.50±0.15) and (26.48±0.73)(μg·h)/ml in aqueous humor, iris-ciliary body, vitreous body, retinal-choroid, cornea and sclera, respectively.Cefuroxime was not detected in the lens at different time points after injection.The parameters of pharmacokinetics were fitted to two compartment model.
Conclusions:Cefuroxime shows good penetration in aqueous humor, iris-ciliary body, vitreous body, retinal-choroid, cornea and sclera when administrated by intravenous injection in rabbits and cefuroxime has no distribution in lens.Cefuroxime can reach an effective concentration in ocular tissues 0.5 to 1.5 hours after intravenous injection.
