Determination of arsenic species compound in blood by high performance liquid chromatographyatomic fluorescence spectrometry
10.3760/cma.j.issn.1001-9391.2019.12.013
- VernacularTitle: 人血中形态砷化合物测定的高效液相色谱-原子荧光光谱法
- Author:
Lezhou ZHOU
1
;
Lu YANG
;
Sheng FU
Author Information
1. Hunan Prevention and Treatment Institute for Occupational Disease, Changsha 410007, China
- Publication Type:Journal Article
- Keywords:
Chromatography, liquid;
Spectrometry, fluorescence;
Arsenic species;
Blood;
Acute intoxication
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2019;37(12):928-931
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for determination of arsenic species in blood with high performance liquid chromatography-atomic fluorescence spectrometry (HPLC-AFS) .
Methods:The effect of mobile phase about chromatography separation and sample pretreatment conditions and atomic fluorescence spectrometry detection parameters has been optimized to reliably measure the following four kinds of species arsenic compound including arsenic[As (III) ]、dimethylarsinic acid (DMA) 、monomethylarsonic acid (MMA) and arsenate[As (V) ] in acute intoxication human blood. The method of technical standard about within-run, between-run and recoveries of standard were optimized.
Results:The method showed As (III) linear relationship was 2.63-100.00 μg/L, The detection limit was 2.63 μg/L. The relative coefficient (r) was 0.9999; DMA linear relationship was 3.21-100.00 μg/L, The detection limit was 3.21 μg/L. The r was 0.9992; MMA linear relationship was 3.41-100.00 μg/L, The detection limit was 3.41 μg/L. The r was 0.9998; As (V) linear relationship was 3.90-100.00 μg/L, The detection limit was 3.90 μg/L. The r was 0.9996. The average recovery of four species arsenic in tested samples ranged from 91.3%-99.8% with the relative standard deviation (RSD) from 2.39% to 4.05%. The within-run and between-run relative standard deviations (RSD) of repetitive measurement at 10.00, 40.00, 80.00 μg/L concentration levels were 1.99%-4.59% and 2.72%-4.53%.
Conclusion:This method is low detection limit, good accurate and high sensitivity, proposed method had been applied to the analysis of arsenic species in blood samples those who acute intoxication or poisoning diagnosis.
