Study on the mechanism of llinc00052 regulating Wnt pathway affecting invasion and metastasis of gastric cancer
10.3760/cma.j.issn.1008-6706.2020.02.011
- VernacularTitle: llinc000522调控Wnt通路影响胃癌侵袭转移的机制研究
- Author:
Guoqing YU
1
;
Yongjin HE
1
;
Difeng XU
1
;
Yong XU
1
Author Information
1. Department of the Second Surgery, Dajiangdong Hospital of Hangzhou, Hanghzou, Zhejiang 311225, China
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Neoplasm lnvasiveness;
Neoplasm metastasis;
Wnt proteins;
Long-chain non-coding RNA;
Plasmids;
Transfection;
Clone cells
- From:
Chinese Journal of Primary Medicine and Pharmacy
2020;27(2):174-178
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of llinc00052 regulating Wnt pathway affecting invasion and metastasis of gastric cancer.
Methods:SGC-7901 cells were selected from gastric cancer cell lines, and siRNAs related to INC0005 and had invasion and metastasis effects in gastric cancer cells were screened by binding of IncRNA expression profiles to qRT-PCR.Ilinc00052 and miRNA expression changes were studied by in vitro cell transfection experiments.Through molecular experiments, the expression of llinc00052 and the effect on Wnt/β-catenin expression were investigated to explore whether llinc00052 could affect the invasion and metastasis of gastric cancer cells by regulating miRNAs affecting Wnt/β-catenin signaling pathway.
Results:Transwell chamber test showed that the number of transmembrane cells in the untransfected plasmid group was (134.10±4.29), and the number of transmembrane cells in the overexpressed llinc000522 plasmid group was (169.24±6.99)(t=8.956, P=0.001). The scratch test showed that the migration distance in the llinc000522 overexpression transfection plasmid group was significantly higher than that in the no-load plasmid transfection group(r=0.907, P<0.01). The clone formation rate of llinc000522 overexpressed transfected plasmid group was significantly higher than that of the empty plasmid group[(92.75±6.32)% vs.(73.34±9.14)%](t=5.998, P<0.05). Compared with the transfection of blank plasmid, the expressions of Wnt1, Wnt3a, Wnt2 and β-catenin mRNA in the llinc000522 overexpression transfection group were significantly up-regulated(P<0.05), while the miRNA transfection group had no significant effect on the expression.The expressions of Wnt1, Wnt3a, Wnt2, and β-catenin mRNA were significantly increased [(1.82±0.11), (1.52±0.15), (1.42±0.21), (1.71±0.19)](P<0.05), but their expressions were still lower than those of the genes transfected with llinc000522 alone.Compared with the blank plasmid transfection, the expressions of Wnt1, Wnt3a, Wnt2 and β-catenin protein in the llinc000522 overexpression transfection group were significantly up-regulated(all P<0.05), while the miRNA transfection group had no significant effect on its expression.The protein expressions of Wnt1, Wnt3a, Wnt2 and β-catenin were also significantly increased[(1.53±0.09), (1.4±0.21), (1.33±0.07), (1.47±0.19)](P<0.05), but their expressions were still lower than those of the gene transfected with llinc000522 alone.
Conclusion:In gastric cancer cells, llinc00052 can affect the invasion and metastasis of gastric cancer by regulating the level of miRNA and affecting the Wnt/β-catenin pathway.
