Molecular epidemiology of Listeria monocytogenes isolated from ready-to-eat food in 2017 in China
10.3760/cma.j.issn.0253-9624.2020.02.012
- VernacularTitle: 2017年中国即食食品中单核细胞增生李斯特菌的分子流行病学特征
- Author:
Weiwei LI
1
;
Yunchang GUO
1
;
Li ZHAN
2
;
Guozhu MA
3
;
Zushun YANG
4
;
Chengwei LIU
5
;
Zhixin SHEN
6
;
Di WANG
7
;
Xiaoai ZHANG
7
;
Xiaohong SONG
8
;
Bo YU
9
;
Huayun JIA
10
;
Xiugui LI
11
;
Xiuli ZHANG
12
;
Xiaorong YANG
13
;
Dajin YANG
1
;
Xiaoyan PEI
1
Author Information
1. Department of Risk Surveillance, China National Center for Food Safety Risk Assessment, Beijing 100022, China
2. Microbiology Laboratory, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China
3. Institute of Pathogen Microbiology and Bio-Testing, Shaanxi Provincial Center for DiseaseControl and Prevention, Xian 710054, China
4. Division of Health Inspection, Yunnan Provincial Center for Disease Control and Prevention, Kunming 650034, China
5. Institute of Nutrition and Food Safety, Jiangxi Provincial Center for Disease Control and Prevention, Nanchang 330029, China
6. Microbiology Laboratory, Hebei Provincial Center for Disease Control and Prevention, Shijiazhuang 050024, China
7. Institute of Nutrition and Food Safety, Beijing Provincial Center for Disease Control and Prevention, Beijing 100013, China
8. Division of disinfection Surveillance, Shanxi Provincial Center for Disease Control and Prevention, Taiyuan 030012, China
9. Institute of Health Inspection, Hubei Provincial Center for Disease Control and Prevention, Wuhan 430079, China
10. Microbiology Laboratory, Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, China
11. Microbiology Laboratory, Guangxi Provincial Center for Disease Control and Prevention, Nanning 530028, China
12. Institute of Health Inspection, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450046, China
13. Microbiology Laboratory, Sichuan Provincial Center for Disease Control and Prevention, Chengdu 610044, China
- Publication Type:Journal Article
- Keywords:
Listeria monocytogenes;
Molecular epidemiology;
Genome-wide sequencing;
Core genome multilocus sequence typing;
Ready-to-eat food
- From:
Chinese Journal of Preventive Medicine
2020;54(2):175-180
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the molecular characteristics of Listeria monocytogenes strains from ready-to eat food in China.
Methods:A total of 239 Listeria monocytogenes strains isolated from ready-to-eat food in 2017, all strains underwent whole-genome sequencing (WGS) , and comparisons uncovered population structure derived from lineages, clonal complex, serogroups, antimicrobial susceptibility and virulence, which were inferred in silico from the WGS data. Core genome multilocus sequence typing was used to subtype isolates.
Results:All strains were categorized into three different lineages, lineage Ⅱ was the predominant types in food, and IIa was the main serogroups. CC8, CC101 and CC87 were the first three prevalent CCs among 23 detected CCs, accounting for 49.4%. Only 4.6% (11 isolates) of tested strains harbored antibiotic resistance genes, which were mostly trimethoprim genes (7 isolates, 2.9%). All strains were positive for LIPI-1, and only a part of strains harbored LIPI-3 and LIPI-4, accounting for 13.8% (33 isolates) and 14.2% (34 isolates), respectively. ST619 carried both LIPI-3 and LIPI-4. 51.5% (123 isolates) of strains carried SSI-1, and all CC121 strains harbored SSI-2. Different lineages, serogroups and CCs can be separated obviously through cgMLST analysis, and 24 sublineages were highly concordant with CCs.
Conclusion:Ⅱa was the main serogroups in ready-to-eat food isolates in China; CC8, CC101 and CC87 were the prevalent CCs, and CC87 isolates was hypervirulent isolates, cgMLST method can be adopted for prospective foodborne disease surveillance and outbreaks detection.
