Research progress of pretargeting strategies in antibody radiolabeling
10.3760/cma.j.issn.2095-2848.2020.02.013
- VernacularTitle: 预靶向策略在抗体放射性标记中的研究进展
- Author:
Huimin ZHOU
1
;
Xiaohua ZHU
Author Information
1. Department of Nuclear Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
- Publication Type:Review
- Keywords:
Radioimmunodetection;
Isotope labeling;
Antibodies;
Trends
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2020;40(2):118-121
- CountryChina
- Language:Chinese
-
Abstract:
Pretargeting is an imaging strategy different from the imaging of traditional radiolabeled antibody, in which the antibody and radioisotope are administered in turn and then combined by coupling system within the body. Due to the high specificity and affinity between the coupling systems, the radioisotope is localized to the target in vivo, and then the radioactive labeling is achieved for imaging. Nuclear imaging using pretargeting methods can decrease the circulation duration of the radioisotope so as to reduce the uptake of the radioactivity in normal tissues, and facilitate the use of short-lived radioisotopes. This article provides a bird′s-eye view of 4 main strategies: biotin-streptavidin (SA) system, bispecific antibodies (BsAb) system, complementary oligonucleotides sequence, and bioorthogonal click chemistry system.
