Effect of uncoupling protein 2 gene on radiation sensitivity of Siha cells
10.3760/cma.j.issn.0254-5098.2020.02.003
- VernacularTitle: 解耦联蛋白2对Siha细胞辐射敏感性的影响
- Author:
Cuihua LIU
1
;
Xinyu DONG
;
Yuanhang LI
;
Xinqiang ZHANG
;
Zhicheng WANG
;
Gang ZHAO
;
Yannan SHEN
Author Information
1. Key Laboratory of Radiobiology, National Health and Health Commission, College of Public Health, Jilin University, Changchun 130021, China
- Publication Type:Journal Article
- Keywords:
Cervical cancer;
RNA interference;
Uncoupling protein 2;
Apoptosis;
Radiation sensitivity
- From:
Chinese Journal of Radiological Medicine and Protection
2020;40(2):88-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether silencing UCP2 can sensitize cervical cancer cell line Siha to radiation.
Methods:Siha cells were transfected with UCP2 siRNA and then irradiated by X-ray. The radiosensitivity of Siha cells was verified by colony formation, CCK-8, apoptosis and immunofluorescence assays. The mitochondrial membrane potential and the production of reactive oxygen species (ROS) were detected to further explore the related mechanism.
Results:RT-PCR and Western blot assays showed that the expression of UCP2 in Siha cells was increased after irradiation and the UCP2 siRNA successfully silenced the expression of UCP in cells. According to the survival curves, the D0, Dq, N and SF2 were 1.54, 1.31, 2.31 Gy and 0.52 for siUCP2 group, 2.50, 3.64, 4.30 Gy and 0.83 for blank control group, and 3.34, 2.16, 1.91 Gy and 0.69, for siNC group, respectively. The radiosensitivity enhancement ratio of silent group was 0.62 and 0.46, compared with blank control group and negative control group, respectively. The proliferative activity of cells in the silent group was lower than that in the control group (t=13.2, P<0.05). Apoptosis levels in the silent group were significantly higher than those in the control group after irradiation(t=3.14, P<0.05). At 4 h after irradiation, the ROS production in the silent group was significantly higher than that in the control group (t=19.10, P<0.05). At 24 h after irradiation, the mitochondrial membrane potential of Siha cells in the silent group was significantly lower than that in the control group (t=4.18, P<0.05).
Conclusions:The radiosensitivity of Siha cells is enhanced after UCP2 silencing, and thus UCP2 may applicable as a new target for radiosensitization of cervical cancer cells.
