Glucagon-like peptide-1 and gastrin synergistically promote the differentiation of insulin-producing cells
10.3760/cma.j.issn.1000-6699.2020.02.010
- VernacularTitle: 胰升糖素样肽1和胃泌素协同促进胰岛素分泌细胞分化
- Author:
Shufang YAN
1
;
Wuyan PANG
1
;
Huijuan YUAN
2
Author Information
1. Department of Endocrinology and Metabolism, Diabetes Research Center, Huaihe Hospital of Henan University, Kaifeng 475000, China
2. Department of Endocrinology and Metabolism, Diabetes Research Center, the People′s Hospital of Henan province, Zhengzhou 450003, China
- Publication Type:Journal Article
- Keywords:
Glucagon-like peptide 1;
Gastrin;
Pancreatic duodenal homeobox 1;
Neurogenin 3;
V-type tendon fibrosarcoma oncogene homologous gene A;
Insulin-producing cells
- From:
Chinese Journal of Endocrinology and Metabolism
2020;36(2):145-149
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Glucagon-like peptide-1(GLP-1) and gastrin synergistically promote the differentiation of insulin-producing cells which differentiated from rat bone marrow mesenchymal stem cells (BMSCs).
Methods:(1)Prepare IPCs model: pancreatic duodenal homeobox 1 (Pdx-1), neurogenin 3 (Ngn3) combined with V-type tendon fibrosarcoma oncogene homolog A (MafA) co-transfected BMSCs differentiation into IPCs; (2)IPCs were divided into 4 groups: Group A(uninduced group), group B(GLP-1 induction group), group C(gastrin induction group), and group D(GLP-1 combined with gastrin induction group). Cultured in high glucose medium for 7 days, the expression levels of insulin2, Pdx-1, GK, nestin, and glucagon mRNA were detected by RT-PCR. The insulin secretion of each group was detected by ELISA.
Results:After cultured for 7 days under high glucose conditions, the morphology of IPCs in each induction group changed significantly, gradually aggregated and formed scattered cell masses, and the combined induction group formed large cell masses. The staining of disulfide brown was reddish brown; The levels of insulin secretion increased gradually on the 0, 3rd, 5th, 7th, and 9th day after induction, and the increase was the most significant in the combined induction group (P<0.05). Compared with group A, the expression of insulin2 and GK in group B and D was significantly up-regulated, the expression of glucagon was down-regulated in group D, the expression of Pdx-1 was down-regulated in group C, and the expression of glucagon was up-regulated (P<0.05). Compared with group B, The expression of insulin2 was down-regulated in group C, and the expression level of glucagon was up-regulated. The expression levels of Pdx-1 and Insulin2 were significantly up-regulated in group D, and the expression level of glucagon was down-regulated (P<0.05). Compared with group C, the expression level of Pdx-1, insulin2 and GK was significantly up-regulated in group D, and the expression level of glucagon was down-regulated (P<0.05).
Conclusion:GLP-1 and gastrin synergistically promote the differentiation of IPCs into islet β cells by up-regulating GK and insulin2 and down-regulating glucagon.
