Effects of miR-373 on proliferation, invasion and migration of osteosarcoma cells
10.3760/cma.j.issn.1008-1372.2020.01.023
- VernacularTitle: miR-373对骨肉瘤细胞增殖、侵袭和迁移的影响
- Author:
Xiaoqian TAN
1
;
Haibo MEI
;
Kun LIU
;
Jin TANG
;
Jiangyan WU
;
Guanghui ZHU
;
Weihua YE
Author Information
1. Department of Orthopedics, Hunan Children′s Hospital, Changsha 410007, China
- Publication Type:Journal Article
- Keywords:
Osteosarcoma;
Cell line, tumor;
miR-373;
Cell proliferation;
Cell invasion;
Cell migration
- From:
Journal of Chinese Physician
2020;22(1):91-95
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the expression of miR-373 in osteosarcoma cells and explore its effects on cell proliferation, invasion, and migration.
Methods:Human osteosarcoma cell line SJSA-1 and human osteoblast hFOB 1.19 cultured in vitro. Human osteosarcoma cell line SJSA-1 were randomly divided into four groups: blank control group (CK group), negative control group (NC group), miR-373 over-expressed plasmid group (miR-373 mimic group) and miR-373 inhibited plasmid group (miR-373 inhibitor group). Cells were transfected with FuGENE® HD transfection reagent. After 48 hours of transfection, the relative expression of miR-373 was detected by quantitative real-time polymerase chain reaction (qRT-PCR), cell proliferation by cell counting kit-8 (CCK-8) and cell invasion and migration by Transwell.
Results:The relative expression of miR-373 in SJSA-1 cells was significantly higher than that in hFOB-1.19 cells (P<0.05); compared with CK group and NC group, the relative expression of miR-373 in the miR-373 mimic group increased significantly (P<0.05), and the relative expression of miR-373 in the miR-373 inhibitor group was significantly lower than that in the control group (P<0.05); optical density (OD) value of miR-373 mimic group at 24 h, 36 h, and 48 h were significantly higher than that of CK group and NC group (P<0.05), and OD value of miR-373 inhibitor group at 24 h, 36 h, and 48 h were significantly lower than that of CK group and NC group (P<0.05); the number of cell migration in the miR-373 mimic group was significantly higher than that in the CK group and NC group (P<0.05), and the number of cell migration in the miR-373 inhibitor group was significantly lower than that in CK group and NC group (P<0.05); the number of cell migration in the miR-373 mimic group was significantly higher than that in the CK group and NC group (P<0.05), and the number of cell migration in the miR-373 inhibitor group was significantly lower than that in CK group and NC group (P<0.05).
Conclusions:The expression of miR-373 is up-regulated in osteosarcoma. Overexpression of miR-373 can promote the proliferation, invasion, and migration of osteosarcoma cells, while the low expression of miR-373 can inhibit the proliferation, invasion, and migration of osteosarcoma cells, which may be an important target for the diagnosis and treatment of osteosarcoma.
