Effects of modified acidic fibroblast growth factor mediated by nanoliposomes combined with ultrasound-targeted microbubble destruction on left ventricular systolic function in diabetic rats
10.3760/cma.j.issn.1004-4477.2020.01.013
- VernacularTitle: 纳米脂质体结合微泡靶向介导改构型酸性成纤维细胞生长因子对糖尿病大鼠左心室收缩功能的影响
- Author:
Lei ZHENG
1
;
Chuanli SHEN
2
;
Yingzheng ZHAO
3
;
Xianwei NI
4
;
Jianmin LI
5
;
Ning YAN
1
;
Xinqiao TIAN
1
Author Information
1. Department of Ultrasonography, Zhengzhou University People′s Hospital(Henan Provincial People′s Hospital), Central China Fuwai Hospital, Zhengzhou 450003, China
2. Department of Ultrasonography, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China
3. School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China
4. Department of Ultrasonography, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
5. Department of Pathology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China
- Publication Type:Journal Article
- Keywords:
Ultrasound-targeted microbubble destruction;
Diabetic cardiomyopathy;
Ventricular function, left;
Nanoliposomes;
Modified acidic fibroblast growth factor
- From:
Chinese Journal of Ultrasonography
2020;29(1):70-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of modified acidic fibroblast growth factor (MaFGF) mediated by nanoliposomes combined with ultrasound-targeted microbubble destruction (UTMD) on left ventricular systolic function in early diabetes mellitus(DM) rats.
Methods:The nanoliposomes containing MaFGF(MaFGF-nlip) were prepared by reverse phase evaporation method. Among 60 male Sprague Dawley (SD) rats, 50 rats were randomly selected and were induced to be DM models by streptozotocin(STZ) through intraperitoneal injecting, the other 10 rats as control group. Then DM rats were randomly divided into 4 groups: DM model group, MaFGF solution group, MaFGF-nlip group and MaFGF-nlip+ UTMD group. After the successful induction of DM model, the intervention was performed twice a week.After 12 weeks of intervention, all rats underwent conventional echocardiography and velocity vector imaging (VVI). Left ventricular ejection fraction (LVEF) and left ventricular fraction shortening(LVFS) were measured by conventional echocardiography. The mean peak systolic radial velocity (Vs), radial strain (Sr) and radial strain rate (SRr) of six walls at the papillary muscle level were measured in left ventricular short-axis view by VVI. At last, myocardial tissue of all rats were stained with Sirius red to evaluate myocardial interstitial fibrosis. The level of myocardial apoptosis was evaluated by TUNEL staining, and the changes of myocardial ultrastructure were observed by transmission electron microscopy.
Results:The prepared MaFGF-nlip were more rounded, evenly dispersed, and of good stability and high encapsulation efficiency. Twelve weeks later after intervention, LVEF, LVFS, Vs, Sr and SRr in the DM model group were significantly lower than those in the control group (all P<0.05). LVEF, LVFS, Vs, Sr and SRr in the MaFGF-nlip+ UTMD group were significantly higher than those of the DM model group and other intervention groups (all P<0.05). The results of Sirius red staining and Tunel staining showed that CVF and AI in the DM model group were significantly higher than those in the control group (all P<0.05). For MaFGF-nlip+ UTMD group, CVF and AI were significantly decreased compared with the DM model group and other intervention groups(all P<0.05). According to the results of transmission electron microscopy, compared with the DM model group, the improvement of myocardial ultrastructure was the most obvious in the MaFGF-nlip+ UTMD group.
Conclusions:MaFGF delivered by using nanoliposomes combined with UTMD can improve the left ventricular systolic function in diabetic rats by inhibiting the myocardium cardiac fibrosis and reducing the cardiomyocyte apoptosis.
