Serum-and-glucocorticoid-inducible-kinase-2 is overexpressed and mediates glycogen synthase kinase-3β/ β-catenin signal transduction in hepatocellular carcinoma
10.3760/cma.j.issn.1007-3418.2020.01.011
- VernacularTitle: 血清和糖皮质激素诱导的蛋白激酶2在肝细胞癌中过表达并介导肝癌细胞中糖原合成酶激酶-3β/β-连环蛋白信号传导
- Author:
Junying LIU
1
;
Xin KONG
1
;
Han LI
1
;
Guili FAN
1
;
Yang LIU
1
;
Zulan LIANG
1
;
Linghui WANG
1
;
Yu ZHANG
1
;
Yuan ZHAO
1
;
Yumei FAN
1
;
Bin WU
1
Author Information
1. Zhoukou Central Hospital, Zhoukou 466000, China
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Serum/glucocorticoid-regulated kinase 2;
Phosphatidylinositol-3-kinase;
Glutathione synthase kinase-3β
;
β
-catenin
- From:
Chinese Journal of Hepatology
2020;28(1):43-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the differential expression of serum-and-glucocorticoid-inducible-kinase-2 (SGK2) in hepatocellular carcinoma (HCC) and normal liver tissues and the related mechanism mediating signal transduction of GSK-3 β / β catenin in HCC cells.
Methods:Twenty pairs of matched HCC and normal tissues were collected and the situation of expression of SGK2 mRNA was detected by real-time fluorescence quantitative PCR. Western blot was used to detect the levels of SGK2 protein in human HCC cell lines (Huh-7, SMMC-7721) and normal human liver cell line (L02). SGK2 siRNA was used to transfect human HCC cell lines (SMMC-7721 and Huh-7), and then the protein expression levels of GSK-3 β/ β - catenin was successfully detected with the above-mentioned transfected cell line by western blot. Measurement data were expressed as mean ± standard deviation (±s), and the Student t -test was used as the statistical method.
Results:SGK2 mRNA expression was up-regulated in all 20 HCC samples than that of the expression of matched normal liver tissues. SGK2 protein levels were significantly higher in Huh-7 and SMMC-7721 than normal human liver cell lines (P < 0.01). The downregulation of SGK2 expression in human HCC cell lines (SMMC-7721 and Huh-7) had inhibited the expression of unphosphorylated GSK-3 β. In addition, the downregulation of SGK2 expression in HCC cell lines had decreased the dephosphorylation of β - catenin to prevent degradation of the β - catenin proteasome.
Conclusion:SGK2 is overexpressed in HCC and mediates GSK-3β/β- catenin signaling in HCC cells.
