Berberine alleviates cerebral ischemia-reperfusion injury in type 2 diabetic rats by inhibiting endoplasmic reticulum stress-related inflammatory pathways
10.3760/cma.j.issn.0254-9026.2020.01.014
- VernacularTitle: 小檗碱抑制内质网应激炎症通路对2型糖尿病大鼠局部脑缺血再灌注损伤的探讨
- Author:
Chong LIU
1
,
2
;
Xu YANG
3
;
Na SHEN
2
;
Yanbo DI
2
;
Xuan LIU
2
;
Qiang CHEN
4
;
Feng HE
5
;
Huanming LI
5
Author Information
1. Department of Anesthesiology, Tianjin Fourth Central Hospital, The Fourth Center Clinical College of Tianjin Medical University, The Fourth Central Hospital Affiliated to Nankai University, Tianjin 300140, China
2. Central Laboratory, Tianjin Fourth Central Hospital, The Fourth Center Clinical College of Tianjin Medical University, The Fourth Central Hospital Affiliated to Nankai University, Tianjin 300140, China
3. Department of Medicine, Tianjin Medical College, Tianjin 300222, China
4. Chinese Medicine, Tianjin Fourth Central Hospital, The Fourth Center Clinical College of Tianjin Medical University, The Fourth Central Hospital Affiliated to Nankai University, Tianjin 300140, China
5. Cardiology, Tianjin Fourth Central Hospital, The Fourth Center Clinical College of Tianjin Medical University, The Fourth Central Hospital Affiliated to Nankai University, Tianjin 300140, China
- Publication Type:Journal Article
- Keywords:
Berberine;
Inflammation;
Diabetes, type 2;
Brain ischemia
- From:
Chinese Journal of Geriatrics
2020;39(1):73-77
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To examine the effects of Berberine(BBR)on inflammatory pathways related to endoplasmic reticulum stress(ERS)in the penumbra area following focal cerebral ischemia-reperfusion injury in type 2 diabetic rats.
Methods:A total of 72 healthy male Sprague-Dawley rats were fed a high-fat, high-sugar diet and injected with streptozotocin to establish a type 2 diabetes mellitus model.The diabetic rats were randomly divided by digital lottery method into a Sham operation group(Sham group), a diabetic rat + BBR treatment group(BBR group), a diabetic middle cerebral artery occlusion(MCAO)model group(MCAO group), and a diabetic rats MCAO + BBR treatment group(MCAO + BBR group). Six rats were included in each group.The two treatment groups received prespecified doses of BBR through gastric perfusion at 48 h, 24 h before surgery, and 6h after surgery.The MCAO model was prepared by a suture occlusion method.The neurological deficit scores were performed, and the expression of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)was detected by enzyme-linked immunosorbent assay(ELISA). The mRNA expression of ERS marker protein GRP78 was detected by quantitative real time polymerase chain reaction(RT-qPCR), and the expression of ERS-related inflammatory pathway proteins[78 Glucoseregulated protein(GRP78)、Pancreatic endoplasmic reticulum Rinase(PERK)、nuclear factor-κB(NF-κB)]was detected by Western blot.
Results:the cerebral ischemic penumbra area, after 2 h of ischemia and 24 h of reperfusion, the neurological deficit score in the MCAO group was higher than that in the MACO+ BBR group [(2.83±0.41)vs.(1.67±0.52), P<0.05], and the expression levels of pro-inflammatory cytokines(TNF-α and IL-1β)and ERS-related inflammatory pathway proteins(GRP78, PERK and NF-κB p65)were also significantly increased(all P<0.05). However, BBR treatment was able to alleviate the neurological dysfunction caused by cerebral ischemia-reperfusion in type 2 diabetic rats(P<0.05). Similarly, BBR treatment also reduced the expression levels of pro-inflammatory factors(TNF-α and IL-1β)and ERS-related inflammatory pathway proteins(GRP78, PERK and NF-κB p65)in the cerebral ischemic penumbra area(all P<0.05).
Conclusions:Through inhibiting ERS-related inflammatory pathways, BBR plays a neuroprotective role to alleviate cerebral ischemia-reperfusion injury in type 2 diabetic rats.
