Expression of NF-kappaB and Cytokines in Chronic Rejection of Transplanted Murine Heart.
10.3346/jkms.2001.16.4.397
- Author:
Jeong Ryul LEE
1
;
Chul Jun SEOK
;
Joon Seok KIM
;
Ji Min CHANG
;
Jeong Wook SEO
Author Information
1. Department of Thoracic and Cardiovascular Surgery, Seoul National University Hospital. jrl@plaza.snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
NF-kappa B;
Cytokines;
Heart Transplantation;
Coronary Arteriosclerosis;
Graft Rejection
- MeSH:
Animal;
Chronic Disease;
Coronary Arteriosclerosis/etiology/*metabolism;
Cytokines/*biosynthesis;
*Graft Rejection;
*Heart Transplantation;
Histocompatibility Antigens Class I/analysis;
Intercellular Adhesion Molecule-1/biosynthesis;
Interferon Type II/biosynthesis;
Mice;
NF-kappa B/*biosynthesis;
Transplantation, Homologous;
Tumor Necrosis Factor/biosynthesis;
Vascular Cell Adhesion Molecule-1/biosynthesis
- From:Journal of Korean Medical Science
2001;16(4):397-406
- CountryRepublic of Korea
- Language:English
-
Abstract:
The heart transplantation-associated accelerated graft arteriosclerosis (AGAS) is one of the major causes of cardiac allograft failure. We investigated the early time-course of expresssion patterns of cytokines, transcription factor, and its inhibitor in the intraabdominally transplanted mice hearts that differed only in the D locus of class I histocompatibility antigen. The allograft hearts were harvested at 1-3, 5, 7, 14, 28, and 42 days after the transplantation, and the expressions of NF-kappaB/I-kappaB and cytokines (TNF-alpha , INF-gamma) were examined in these specimens. The expressions of TNF-alpha and INF-gamma were observed on day 1, peaking on day 5 and 7, respectively. Activated NF-kappaB (p65) expression was present on the cytoplasm and perinuclear area in the endothelial cells of coronary arteries on day 1. The peak of translocation of NF-B from cytoplasm to nucleus appeared on day 5 in the endothelial cells, myocytes, and leukocytes within the vessels, and remained elevated until day 42. The I-kappaB expression gradually increased from day 1 until day 5, but a remarkable decrease was detected on day 7. Our data suggest that the increased expressions of NF-kappaB/I-kappaB and cytokines (TNF-alpha, INF-gamma) play an important role in inducing immune responses in the donor allograft heart and hence the blockage of the expressions might be mandatory to avoid a potential graft failure.
