Apoptosis of Skeletal Muscle on Steroid-Induced Myopathy in Rats.
10.3346/jkms.2001.16.4.467
- Author:
Myung Ju LEE
1
;
Ji Shin LEE
;
Min Cheol LEE
Author Information
1. Department of Plastic and Reconstructive Surgery, College of Medicine, Chosun University. mclee@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Myopathy, Steroid-Induced;
Apoptosis;
Fas Antigens, CD95;
Protein p53;
Muscular Diseases
- MeSH:
Animal;
Antigens, CD95/analysis;
*Apoptosis;
Female;
Immunohistochemistry;
Microscopy, Electron;
Muscle, Skeletal/*pathology/ultrastructure;
Muscular Diseases/chemically induced/*pathology;
Protein p53/analysis;
Rats;
Rats, Sprague-Dawley;
Triamcinolone Acetonide/*toxicity
- From:Journal of Korean Medical Science
2001;16(4):467-474
- CountryRepublic of Korea
- Language:English
-
Abstract:
Recently apoptotic cell death has been reported in differentiated skeletal muscle, where apoptosis was generally assumed not to occur. To investigate whether apoptosis may contribute to the steroid-induced myopathy, rats treated with triamcinolone acetonide (TA) for 9 days were sacrificed for detecting apoptosis by in situ end labeling (ISEL) and electron microscopy in the soleus muscles. Immunohistochemical stainings of Fas antigen and p53 protein were performed to examine whether apoptosis-related proteins were present in the myopathy. Muscle fiber necrosis and apoptotic myonuclei appeared in the soleus muscles following administration of TA, while control muscles showed no evidences for apoptosis. Fas antigen was not detected in control muscles, but expressed in the soleus muscles of steroid-induced myopathy. Some of the Fas antigen-expressing muscle fibers were positive for ISEL. p53 protein was not detected in any muscle fibers. These findings indicate that TA can induce apoptosis in differentiated skeletal muscles, and Fas antigen might be partly related to apoptotic muscle death in steroid-induced myopathy.
