Research progress of diagnosis platforms based on CRISPR/Cas system
10.3760/cma.j.issn.1009-9158.2020.01.012
- VernacularTitle: 基于CRISPR/Cas系统诊断平台的研究进展
- Author:
Xiaoyu GU
1
;
Guihua WANG
;
Shaoqing JU
;
Xudong WANG
Author Information
1. Department of Clinical Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong 226001, China
- Publication Type:Review
- Keywords:
CRISPR-Cas systems;
CRISPR-associated proteins;
Endonucleases;
Sensitivity and specificity
- From:
Chinese Journal of Laboratory Medicine
2020;43(1):96-100
- CountryChina
- Language:Chinese
-
Abstract:
At present, nucleic acid testing technology has been widely used in clinical laboratory diagnosis. Conventional detection technique such as real-time PCR is complicated, time consuming, and dependent on specific instruments. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas) system is an adaptive immune defense system against viruses in bacteria and archaea, which has been developed into a powerful technology for genome editing. Recently, the leading groups engaged in CRISPR have set up new tools for nucleic acid detection based on Cas13a, Cas12a and newly discovered protein-Cas14, which plays an important role in rapid diagnosis of infectious diseases, detection of gene mutations in cancer and genotyping. Since they are ultrasensitive, specific, rapid and cost-effective, it is expected to bring great potential for molecular diagnosis. In this review, the mechanism of CRISPR/Cas system and the principle, the applications of the newly-developed diagnostic platforms are introduced. What′s more, the advantages, limitations and prospects of the technologies are summarized.
