The functions of miR-3960 in all-trans-retinoic acid induced cleft palate mouse model
10.3760/cma.j.issn.1009-4598.2020.01.016
- VernacularTitle: 微小RNA-3960在过量全反式维甲酸诱导腭裂小鼠模型中的作用
- Author:
Chao WEI
1
;
Haitao HUANG
2
;
Jing XIAO
1
;
Ru WANG
2
Author Information
1. Department of Oral Pathology, College of Stomatology, Dalian Medical University, Dalian 116044, China
2. Department of Stomatology, First Affiliated Hospital of Dalian Medical University, Dalian 116023, China
- Publication Type:Journal Article
- Keywords:
All-trans-retinoic acid;
Cleft palate;
miR-3960;
Gene;
Bioinformatics
- From:
Chinese Journal of Plastic Surgery
2020;36(1):72-75
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To research the functions of miR-3960 in all-trans-retinoic acid (atRA) induced cleft palate mouse model in order to provide the theoretical basis for gene therapy of cleft palate.
Methods:Excessive atRA induced cleft palate mouse model was established and palatine process tissues were collected. qRT-PCR was used to detect the expression of miR-3960. miRBase was used to analyse the characteristics of miR-3960 sequence. TargetScanMouse Prediction of microRNA targetswas used to predict the target genes of miR-3960. DAVID v6.8 database was used to perform bioinformatics analysis of target genes.
Results:miR-3960 was up-regulated in the experimental group. From the analysis of miRBase, we only got the sequences of miR-3960 in two species, and the two sequences were the same. There were 320 predicted target genes, the functions were mainly concentrated in cell proliferation, cell differentiation, embryo development and tissue development and so on (P<0.05), the signaling pathways were mainly concentrated in the calcium signaling pathway, cAMP signaling pathway and cGMP-PKG signaling pathway and so on (P<0.05).
Conclusions:In excessive atRA induced cleft palate mouse models, the up-regulated miR-3960 may result in cleft palate by inhibiting the proliferation and differentiation of palatal mesenchymal cells.
