Clostridium difficile toxin B-induced apoptosis of colon cancer cells and related mechanisms
10.3760/cma.j.issn.0254-5101.2020.01.010
- VernacularTitle: 艰难梭菌毒素B诱导结肠癌细胞凋亡及相关机制研究
- Author:
Xiaolang HUANG
1
;
Chengjian WANG
;
Wuyingga BAO
;
Henan CAI
;
Qing PENG
;
Siwu FU
Author Information
1. Medical College of Northwest Minzu University, Lanzhou 730030, China
- Publication Type:Journal Article
- Keywords:
Clostridium difficile toxin B;
Colon cancer SW480 cells;
Proliferation;
Apoptosis
- From:
Chinese Journal of Microbiology and Immunology
2020;40(1):55-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the effects of Clostridium difficile toxin B (TcdB) on the proliferation and apoptosis of colon cancer cell line SW480 and the possible mechanisms related to cell apoptosis.
Methods:SW480 cells were treated with different concentrations of TcdB. Cell proliferation was detected by MTT assay. Cell apoptosis and mitochondrial membrane potential were measured with flow cytometry.
Results:TcdB significantly inhibited the proliferation of SW480 cells in a time-concentration dependent manner and the inhibition rate reached 46.36% at 48 h. Flow cytometry results showed that TcdB could induce the apoptosis of SW480 cells in a time-concentration dependent manner and a 20.83% apoptosis rate was induced by 800 ng/ml of TcdB at 48 h.
Conclusions:TcdB could inhibit the proliferation and induce the apoptosis of colon cancer SW480 cells, and the possible mechanisms might be relate to the initiation of mitochondrial apoptosis pathway.
