Isolation and identification of Prevotella nigrescens in patients with chronic periodontitis and analysis of its tumorigenic role in esophageal squamous carcinogenesis
10.3760/cma.j.issn.0254-5101.2020.01.009
- VernacularTitle: 慢性牙周炎口腔变黑普雷沃氏菌分离鉴定及促进食管鳞癌演进
- Author:
Qiwei LIU
1
;
Yelin JIAO
;
Haojie RUAN
;
Pan CHEN
;
Ke LIU
;
Mengxiang LI
;
Bianli GU
;
Shegan GAO
;
Yijun QI
Author Information
1. Henan Key Laboratory of Cancer Epigenetics, Cancer Hospital, the First Affiliated Hospital, College of Clinical Medicine, Medical College of Henan University of Science and Technology, Luoyang 471003, China
- Publication Type:Journal Article
- Keywords:
Periodontitis;
Prevotella nigrescens;
Isolation;
Identification;
Esophageal squamous cell carcinoma
- From:
Chinese Journal of Microbiology and Immunology
2020;40(1):49-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate and identify Prevotella nigrescens (P.nigrescens) in gingival crevicular fluid of patients with chronic periodontitis and to analyze its tumor-promoting role in esophageal squamous cell carcinoma (ESCC).
Methods:Samples of gingival crevicular fluid were collected from patients with chronic periodontitis and cultured on GAM agar medium under anaerobic conditions. Black colonies on GAM agar plates were picked for subculture, and then the bacteria were isolated and purified. Bacterial identification was conducted using Gram staining and 16S rDNA sequencing analysis. The isolated bacterial species was used to infect ESCC NE6-T cells and the changes in biological characteristics of the infected cells were evaluated.
Results:Multiple bacterial colonies were observed after anaerobic culturing of gingival crevicular fluid samples for 120 h on GAM agar plates. A single bacterial colony with pure black and smooth appearance was obtained from grey black bacterial colonies with streak method. It was a Gram-negative bacterium with bead-like shape. It showed 99.78% 16S rDNA sequence identity with P. nigrescens F0103 and was named P. nigrescens LY01. P. nigrescens LY01 infection promoted the in vitro proliferation, migration and invasion of NE6-T cells and the growth of subcutaneous xenograft in nude mice. In addition, it could also induce the upregulation of Ki67 and activation of p-STAT3.
Conclusions:P. nigrescens inhabiting in gingival sulcus might promote the progression of ESCC in human with chronic periodontitis.
