Role of HIV-1 envelope protein gp120 in neuronal injury-induced cognitive impairment
10.3760/cma.j.issn.0254-5101.2020.01.006
- VernacularTitle: HIV-1包膜蛋白gp120在神经元损伤诱发认知障碍中的作用
- Author:
Yu WAN
1
;
Weijun YANG
;
Zelong GONG
;
Zhijie ZENG
;
Hanyun ZHANG
;
Keyao LYU
;
Hong CAO
Author Information
1. Department of Microbiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China
- Publication Type:Journal Article
- Keywords:
HIV-1;
gp120;
Cognitive impairment;
gp120-transgenic mouse;
Microglia
- From:
Chinese Journal of Microbiology and Immunology
2020;40(1):32-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of HIV-1 envelope protein gp120 in cognitive impairment induced by neuronal damage.
Methods:Western blot and immunofluorescence assay were used to detect microglia activation, inflammatory factor expression and neuronal damage after gp120 treatment. Neuronal damage and neurocognitive performance in gp120-transgenic mice were evaluated using immunohistochemical staining and behavioral analysis, respectively.
Results:In vivo and in vitro experiments showed that HIV-1 gp120 significantly induced the expression of caspase-1 and IL-1β, and indirectly caused neuronal synaptic shortening and neuronal damage (P<0.05). Compared with wild-type mice, gp120-transgenic mice showed significant cortical and hippocampal glial activation, neuronal loss, dendritic damage and neurocognitive disorders.
Conclusions:HIV-1 gp120 might cause neuronal damage through activating the release of inflammatory factor by microglia and involve in neurocognitive impairment.
