Determination of 1-methoxy-2-propanol in urine by headspace solid-phase microextraction coupled with gas chromatography
10.3760/cma.j.issn.1001-9391.2020.01.013
- 白话标题: 尿中1-甲氧基-2-丙醇测定的顶空固相微萃取-气相色谱法
- 作者:
Hongmin GAO
1
;
Shizhen SONG
;
Yong MEI
;
Menghan NIE
;
Ruodan FANG
;
Wanting SU
作者信息
1. School of Public Health, Wuhan University of Science and Technology, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan 430065, China
- 出版类型:Journal Article
- 关键字:
Chromatography, gas;
Solid phase microextraction;
1-methoxy-2-propanol;
Propylene glycol monomethyl ether;
Urine
- 来源:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2020;38(1):52-55
- 国家China
- 语言:Chinese
-
摘要:
Objective:To establish a method for the determination of 1-methoxy-2-propanol in urine using headspace solid phase micro-extraction coupled with gas chromatography.
Methods:The 1-methoxy-2-propanol was enriched by headspace solid phase micro-extraction fiber coated with carbene/polydimethylsiloxane (CAR/PDMS) . Single factor rotation method was used to optimize the conditions of extraction temperature, salt amount, and extraction time. The separation was performed on DB-5 (30 m×0.32 mm×0.25 μm) capillary column and detected with flame ionization detector. The quantification was based on the standard curve.
Results:The concentration of 1-methoxy-2-propanol in urine was linear in the range of 0.50-10.0 mg/L, and the linear correlation coefficient was 0.9993. The detection limit of the method was 0.14 mg/L, and the limit of quantification was 0.45 mg/L. The recovery was 85.8% to 104.7%, and the RSD of intra- and inter-batch precision were 3.25%-6.65% and 0.81%-3.96%, respectively.
Conclusion:The method is high sensitivity and simple operation, and is suitable for the determination of 1-methoxy-2-propanol in urine of occupational exposure population.
