The effect of peroxiredoxin 2 on transforming growth factor-β1-induced fibroblast proliferation and collagen synthesis
10.3760/cma.j.issn.1001-9391.2020.01.002
- VernacularTitle: 硫氧环蛋白过氧化物酶-2对转化生长因子β1诱导的人胚肺成纤维细胞增殖及胶原合成的影响
- Author:
Zhao ZHANG
1
,
2
;
Yingyu LIU
3
;
Yan LIU
1
;
Qian LI
1
;
Tingting LIANG
1
;
Fan HONG
1
;
Li FENG
1
;
Ying SUN
1
,
4
Author Information
1. Department of Pathology, School of Basic Medical Science, North China University of Science and Technology, Tangshan 063210, China
2. Department of Respiratory Medicine, Tangshan Works Hospital Affiliated to North China University of Science and Technology, Tangshan 063000, China
3. Department of Respiratory Medicine, Tangshan Works Hospital Affiliated to North China University of Science and Technology, Tangshan 063000, China
4. Hebei Chronic Disease Key Laboratory Disease, School of Basic Medical Science, North China University of Science and Technology, Tangshan 063210, China
- Publication Type:Journal Article
- Keywords:
Silicosis;
Fibroblasts;
Peroxiredoxin 2;
Reactive oxygen species;
Transforming growth factor beta1
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2020;38(1):7-12
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of peroxiredoxin 2 (Prx2) overexpression on fibroblast proliferation and collagen synthesis induced by transforming growth factor-β1 (TGF-β1) .
Methods:Fibroblasts were randomly divided into control group (DMEM medium) , TGF-β1 group (5 μg/L TGF-β1) , negative control group (treated with 5 μg/L TGF-β1 and transfected with empty lentiviral vector) , and Prx2 group (treated with 5 μg/L TGF-β1 and transfected with Prx2 overexpression lentiviral vector) . MTT assay was used to measure cell proliferation, immunofluorescence assay was used to measure the expression of 8-OHdG, and Western blot was used to measure the expression of p-JNK, p-P38, collagen type I, collagen type III, and Prx2. SPSS 18.0 was used for statistical analysis. The continuous data were expressed as mean±standard deviation; an analysis of variance was used for comparison between groups, and the least significant difference t-test was used for further comparison between two groups.
Results:Lentiviral transfection was performed successfully, and the Prx2 group had a significant increase in the protein expression of Prx2 (P<0.05) . Compared with the control group, the TGF-β1 group had a significant increase in the proliferation ability (P<0.05) , and compared with the TGF-β1 group, the Prx2 group had a significant reduction in the proliferation ability (P<0.05) . Compared with the control group, the TGF-β1 group had significant increases in the expression of 8-OHdG, p-JNK, p-P38, collagen type I, and collagen type III (P<0.05) ; compared with the TGF-β1 group, the negative control group had no significant changes in the expression of 8-OHdG, p-JNK, p-P38, collagen type I, and collagen type III (P>0.05) , while the Prx2 group had significant reductions in the above parameters (P<0.05) .
Conclusion:Prx2 overexpression inhibits fibroblast proliferation and collagen synthesis induced by TGF-β1 through inhibiting reactive oxygen species and activating the JNK and P38 pathways.
