The influence of dihydrotestosterone on cytokine releases in male mice model with Graves disease
10.3760/cma.j.issn.0578-1426.2020.01.008
- VernacularTitle: 二氢睾酮对雄性小鼠Grave病模型淋巴细胞因子的影响
- Author:
Lianye LIU
1
;
Bingyin SHI
;
Liping WU
;
Aibo GAO
Author Information
1. Department of Endocrinology, The First Affiliated Hospital of Xi′an Jiaotong University, Xi′an 710061, China (Liu Lianye is working in Department of Endocrinology, Weinan Central Hospital, Weinan, Shaanxi 714000, China)
- Publication Type:Journal Article
- Keywords:
Graves disease;
Dihydrotestosterone;
Cytokines;
Mice
- From:
Chinese Journal of Internal Medicine
2020;59(1):47-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the influences of dihydrotestosterone (DHT) on the development of experimental autoimmune Graves disease (EAGD), and to observe the effect of DHT on cytokines in male BALB/c mice model.
Methods:Male BALB/c mice aged 6-8 weeks were divided into 4 groups using random number table: (1) control group; (2) EAGD group; (3) placebo group; (4) DHT group. EAGD mice were induced with an adenovirus expressing the human thyroid stimulating hormone receptor antibody A-subunit (Ad-TSHR289). DHT (5mg) or a matching placebo were implanted one week before the first immunization. Thyroid hormones were detected with radioimmunoassay kit.. Cytokines [such as interferonγ (IFNγ), interleukin (IL)-4, IL-10, IL-9, and IL-17] producing cells from the spleen were detected using flow cytometry.
Results:As expected Ad-TSHR289 treatment increased total thyroxine [EAGD group vs. control group: (117.76±32.69) nmol/L vs. (33.08±12.61) nmol/L, P<0.0001] and free thyroxine [EAGD group vs. control group: (15.01±11.55) pmol/L vs. (3.55±1.88) pmol/L, P<0.0001]. Treatment of DHT slightly lowered thyroid hormones [DHT group vs. placebo group: total thyroxine (114.80±44.27) nmol/L vs. (123.17±77.73) nmol/L; free thyroxine (13.48±6.01) pmol/L vs. (14.19±12.65) pmol/L], without significant difference (all P>0.05)]. However, the percentage of IL-10, but not IFN γ, IL-4, IL-9 and IL-17, secreted spleen cells increased in DHT group than in the placebo group [(7.11±3.29)% vs. (3.51±1.36)%, P<0.05].
Conclusion:The effects of DHT on thyroid hormone are mild. It might play an immunomodulatory role in the male mouse Graves disease model by up-regulating the cytokine IL-10.
