Changes of Serum Biomarkers levels in Early Stage of Alcohol-induced Liver Injury in Rats
10.13422/j.cnki.syfjx.20190223
- VernacularTitle: 酒精致大鼠肝损伤早期血清生物标志物水平的变化规律
- Author:
Ping-sheng ZHU
1
;
Yan-jie JIAO
1
;
Shuang-nan FU
1
;
Ming-san MIAO
1
;
Yu MENG
1
;
Da GAO
1
;
Zheng-wang ZHU
1
Author Information
1. Henan University of Chinese Medicine, Zhengzhou 450046, China
- Publication Type:Research Article
- Keywords:
acute liver injury;
alcohol;
early biomarkers;
change law
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(2):129-133
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the dynamic changes of the biomarkers of alcoholic liver injury, including glutamate dehydrogenase(GLDH), α-glutathione-S-transferase(α-GST), purine nucleotide phosphorylase(PNP), and arginine enzyme 1(Arg1), and clarify whether these indexes can be used as early diagnostic biomarkers for alcoholic liver injury. Method:48 Wistar rats were randomly divided into a blank group and a model group, 24 rats in each group, half male and half female. After fasting but except water for 7 h, 50% ethanol/10 mL·kg-1 was given to the model group by intragastric administration and the same volume of normal saline was administered to the blank group. After 1 h, 50% ethanol was again given for once by intragastric administration according to the previous dosage. In the blank group, the same volume of normal saline was administered. After modeling and administration for 6 d, acute alcoholic liver injury model was established. 3 h after the last intragastric administration of alcohol at day 2, 3, 4, 6, six rats (half male and half female) in each group were randomly selected. All the animals were sacrificed to determine the aspartate aminotransferase(AST), alanine aminotransferase(ALT), alkaline phosphatase(ALP), bilirubin(TBIL), GLDH, α-GST, PNP, and Arg1 levels. Result:As compared with the blank group, the levels of ALT, AST, ALP, TBIL, GLDH, PNP, α-GST and Arg1 in the model group were significantly different (P<0.01), indicating that the alcoholic liver injury model was successfully established. In the model group, GLDH, PNP, α-GST and Arg1 levels were increased earlier and more significantly than ALT and AST levels. Conclusion:GLDH, PNP, α-GST and Arg1 can be used as biomarkers for early detection of alcoholic liver injury.
