Analysis of Pharmacological Effect of Total Tannins from Spatholobi Caulis on Cervical Cancer HeLa Cells by 3D Microfluidic Chip
10.13422/j.cnki.syfjx.20182204
- VernacularTitle: 基于3D微流控芯片分析鸡血藤总鞣质对宫颈癌细胞HeLa的药理作用
- Author:
Ni-jia WANG
1
;
Jia-yi WANG
2
;
Xian-sheng MENG
1
;
Yong-rui BAO
1
;
Shuai WANG
1
;
Tian-jiao LI
1
Author Information
1. Liaoning Province Multi-component Chinese Medicine Engineering Technology Research Center, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
2. Liaoning Institute for Drug Control, Shenyang 110023, China
- Publication Type:Research Article
- Keywords:
Spatholobi Caulis;
total tannins;
3D microfluidic chip;
cervical cancer HeLa cells;
cell apoptosis;
cell cycle;
enzyme-linked immunosorbent assay
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(2):103-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study on the chemical constituents of total tannins from Spatholobi Caulis,and to analyze the pharmacodynamics and mechanism of total tannins from Spatholobi Caulis on cervical cancer HeLa cells. Method:UPLC-Q-TOF/MS was used to qualitatively analyze the composition of total tannins from Spatholobi Caulis.The appropriate concentration and time of administration were screened by 3 dimensional(3D) microfluidic chip.Flow cytometry was used to determine the effect of total tannins from Spatholobi Caulis on the cell cycle and apoptosis of cervical cancer HeLa cells and analyzed by FlowJo v10.0.7 and ModFit LT 3.2 software.Enzyme-linked immunosorbent assay(ELISA) was used to determine the changes of vascular endothelial growth factor(VEGF)-A and Caspase-3 factors in cervical cancer HeLa cells supernatant treated with total tannins from Spatholobi Caulis. Result:Total of 15 components in total tannins from Spatholobi Caulis were identified or inferred.The low,medium and high dosages of total tannins from Spatholobi Caulis were 0.5,1.0, 2.0 g·L-1 and the best time of administration was 36 h.The proportions of early and late apoptosis of cervical cancer HeLa cells increased significantly in the apoptosis analysis after being treated by total tannins from Spatholobi Caulis.The DNA synthesis early phase(G0/G1 phase) of cervical cancer HeLa cells significantly increased,and the DNA synthesis phase(S phase) and the DNA synthesis late phase(G2/M phase) reduced.After being treated with total tannins from Spatholobi Caulis,the expression of VEGF-A in cervical cancer HeLa cells supernatant was significantly decreased and the expression of Caspase-3 was significantly increased. Conclusion:Spatholobi Caulis is rich in tannins,which can significantly inhibit the proliferation of cervical cancer HeLa cells and promote its apoptosis.This paper can provide the basis for further research of total tannins from Spatholobi Caulis.
