Effect of Berberine on HCCLM3 Cells Apoptosis and Autophagy Under High Glucose Condition
10.13422/j.cnki.syfjx.20182322
- VernacularTitle: 小檗碱对高糖环境下HCCLM3细胞凋亡与自噬的影响
- Author:
Qian LIU
1
;
Ying FU
1
;
Xiao-fei ZI
1
;
Yao-bin XIONG
2
;
Yuan LI
3
Author Information
1. The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China
2. Jiangxi Academy of Medical Sciences, Nanchang 330006, China
3. Nanchang University Hospital, Nanchang 330006, China
- Publication Type:Research Article
- Keywords:
berberine;
HCCLM3 cells;
high glucose;
apoptosis;
autophagy
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(2):68-73
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the anti-tumor molecular mechanism of berberine (BBR)by observing and analyzing its effect on proliferation, apoptosis and autophagy-related gene expression for HCCLM3 cells under high glucose condition. Method:HCCLM3 cells were added into low, medium or high-concentration groups of glucose. It was found in cell counting kit-8(CCK-8)that the high concentration of glucose had the most obvious effect on HCCLM3 cells proliferation. Based on the above experimental result, HCCLM3 cells treated with high concentration of glucose was selected and then different concentrations of berberine (5, 10, 20, 30, 40, 50 μmol·L-1) was added for in vitro intervention for 24 h. Then the effect of each drug group on the proliferation of HCCLM3 cells were studied. At the same time, the control group of metformin was arranged. After that, the changes of apoptosis rate were observed by flow cytometry, and the expression levels of B-cell lymphoma-2(Bcl-2)and autophagy genes Atg5, Beclin1 were detced by Real-time polymerase chain reaction (Real-time PCR). Result:With the increase of glucose concentration, HCCLM3 cell had the strongest migration and proliferation ability in high glucose group (P<0.05). The inhibitory effect of BBR on the proliferation of HCCLM3 in high glucose culture was correlated with the concentration of BBR(5,10,20,30,40,50 μmol·L-1), and the inhibition rate was 33.86%, 40.75% (P<0.05), 49.22%, 55.1%, 60.12%, and 61.42% respectively from low concentration to high concentration (P<0.01). The apoptosis rate was significantly increased with the increase of BBR concentration (P<0.01). The expression levels of Bcl-2, Atg5 and Beclin1 gene were significantly up-regulated in all intervening groups as compared with those in single high glucose group (P<0.05, P<0.01). Conclusion:BBR could inhibit the proliferation of HCCLM3 cells in high glucose environment. Its inhibition effect for HCCLM3 cells might be achieved by inducing apoptosis of the cells, regulating Bcl-2 and up-regulating the expression levels of autophagy gene Beclin1 and Atg5.Thus BBR plays an anti-tumor role through promoting autophagy in high glucose environment.
