Effect of Mangiferin on mRNA Expressions of Hepatic PRPS, PRPPAT and Cerebral HGPRT in Hyperuricemic Mice Induced by Potassium Oxonate
10.13422/j.cnki.syfjx.20190292
- VernacularTitle: 芒果苷对高尿酸血症小鼠血尿酸及嘌呤代谢相关酶表达的影响
- Author:
Hua LIN
1
;
Li-xian YUAN
2
;
LI-hui GAO
1
;
Ling LI
1
;
Yan-fen NIU
1
Author Information
1. Biomedical Engineering Research Center of Kunming Medical University, Kunming 650500, China
2. Qujing Second People's Hospital of Yunnan Province, Qujing 655000, China
- Publication Type:Research Article
- Keywords:
mangiferin;
hyperuricemia;
phosphoribosylpyrohoosphate synthetase (PRPS);
phosphate ribose pyrophosphate amide transferase (PRPPAT);
hypoxanthine-guanine phosphate transfer enzyme (HGPRT)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(2):55-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of mangiferin on the mRNA expression of phosphoribosylpyrohoosphate synthetase (PRPS), phosphate ribose pyrophosphate amide transferase (PRPPAT) in liver and hypoxanthine-guanine phosphate transfer enzyme (HGPRT) in brain of hyperuricemic mice induced by potassium oxonate. Method:Hyperuricemic mice were induced through intraperitoneal injection with uricase inhibitor potassium oxonate. The serum uric acid level was determined by the phosphotungstic acid method. The mRNA expression levels of PRPS and PRPPAT in liver as well as HGPRT in brain of hyperuricemic mice were measured by reverse transcriptase polymerase chain reaction (RT-PCR). Result:An intraperitoneal injection with potassium oxonate caused a marked increase in serum uric acid level, compared with normal control group (P<0.05). Intragastric administration with mangiferin at the doses of 1.5, 3.0, 6.0 mg·kg-1 was able to significantly reduce serum uric acid levels, compared with hyperuricemic control group (P<0.05,P<0.01); Moreover, the increase in the mRNA expression levels of PRPS and PRPPAT in liver, as well as the decrease in the mRNA expression of HGPRT in brain were observed in hyperuricemic control group, but with no statistically difference. Mangiferin has no impact on the mRNA expressions of PRPS, PRPPAT and HGPRT, compared with hyperuricemic control group. Conclusion:The hyporuricemic effect of mangiferin might not be related with PRPS, PRPPAT and HGPRT in therapeutic dose.
