Effect of Yingyang Gongji Wan Contained Serum on Inhibiting HepG2 Cell Proliferation and Invasion, and Inducing Apoptosis
10.13422/j.cnki.syfjx.20182420
- VernacularTitle: 阴阳攻积丸含药血清抑制HepG2细胞增殖、侵袭及促进其凋亡的作用
- Author:
Yong-wei LI
1
;
He-ping XIE
1
;
Hong-jie CHEN
1
;
Yue LI
1
;
Yan CHEN
1
;
Yi-wei LI
1
;
Yu-jie LI
1
;
Xu XIANG
1
Author Information
1. The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China
- Publication Type:Research Article
- Keywords:
Yingyang Gongji Wan;
primary liver cancer;
HepG2 cell;
epithelial-mesenchymal transition (EMT)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(2):28-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Yingyang Gongji Wan (YYGJ) on hepatoma cell line HepG2, and provide evidence for clinical application. Method:YYGJ-contained rats serum was prepared. Then the inhibiory rate of cells was detected by methye thiazolye telrazlium (MTS) method in both YYGJ group and blank group. Apoptosis of HepG2 was detected by TdT-mediated dUT nick-end labeling (TUNEL) method in blank group,YYGJ group, and 5-fluorouracil (5-FU) group. The mRNA expression and protein expression levels of E-cadherin, Vimentin, metalloproteinase-2 (MMP-2) and Smad4 were detected by Real-time quantitative PCR (Real-time PCR) and Western blot respectively. The invasion ability of HepG2 cells was detected by cell migration assay (transwell). Result:YYGJ-contained serum inhibited the proliferation of HepG2 cells in a time and concentration-dependent manner. As compared with blank group, the inhibitory rate was increased in 5%, 10%, and 20% YYGJ-contained serum groups on the third day (P<0.05), and apoptosis rate was also increased significantly in YYGJ and 5-FU groups (P<0.05), but there was no significant difference between 50% YYGJ group and 5-FU group in apoptosis rate. As compared with blank group, the mRNA and protein expression levels of E-cadherin and Smad4 were increased significantly, while Vimentin and MMP-2 mRNA and protein expression levels were decreased significantly in YYGJ and 5-FU groups (P<0.05). HepG2 cell invasive ability was decreased significantly in YYGJ and 5-FU groups as compared to blank group (P<0.05), but there was no significant difference between 50% YYGJ group and 5-FU group. Conclusion:YYGJ-contained serum can inhibit the proliferation of HepG2 cells, induce apoptosis, regulate epithelial-mesenchymal transition (EMT)-related E-cadherin, Vimentin, MMP-2 and Smad4 genes and proteins, and decrease tumor invasion ability. The effect was similar to that of 5-fluorouracil. As a unique prescription, YYGJ can be used as a representative for the treatment of coldness and dampness syndrome of primary liver cancer and its anti-cancer mechanism should be further studied.
