Effect of Saposhnikoviae Radix-Mume Fructus Drug-containing Serum in Regulating Phenotypic Transformation of Airway Smooth Muscle Cell Proliferation Model
10.13422/j.cnki.syfjx.20190224
- VernacularTitle: 防风-乌梅配伍含药血清对气道平滑肌细胞增殖模型表型转化调控作用
- Author:
Sheng-zhi LONG
1
;
Hai-yan ZHU
2
;
Xian-bo WU
3
;
Lin CHEN
2
;
Yi-qiang XIE
4
;
Fan YANG
4
Author Information
1. Guiyang University of Chinese Medicine, Guiyang 550002, China
2. The First Affiliated Hospital of Chengdu Medical College, Chengdu 610050, China
3. Xindu District Hospital of Traditional Chinese Medicine, Chengdu 610050, China
4. School of Traditional Chinese Medicine, Hainan Medical University, Haikou 571101, China
- Publication Type:Research Article
- Keywords:
Saposhnikoviae Radix;
Mume Fructus;
asthma;
airway smooth muscle cells;
airway remodeling;
cell phenotype
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(2):1-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Saposhnikoviae Radix-Mume Fructus containing-serum in regulating the phenotypic transformation of airway smooth muscle cells (ASMCs) proliferation model, in order to explore the mechanism of combined administration of "Saposhnikoviae Radix, Mume Fructus" in inhibiting airway remodeling, and reveal the compatibility mechanism of traditional Chinese medicine. Method:The proliferation model of ASMCs was established by platelet derived growth factor (PDGF) induction. The rats were given normal saline, Saposhnikoviae Radix-Mume Fructus, Saposhnikoviae Radix-Mume Fructus(15.425, 15.425, 30.85 g·kg-1·d-1) to prepare drug serum respectively. Four generations of logarithmic phase human bronchial smooth muscle cells (HBSMC) were collected and divided into blank control group, cell model group, normal rat serum group and normal rat serum cell model group, hormone intervention group, Saposhnikoviae Radix serum group, Mume Fructus serum group, Saposhnikoviae Radix-Mume Fructus serum group. The cells were given corresponding treatment. Immunofluorescence staining and Western blot were adopted to detect ASMCs deflating marks protein α-actin and osteopontin (OPN) expressions, and phenotypic transformation was observed; the levels of vascular endothelial growth factor(VEGF), transforming growth factor-β(TGF-β) and interleukin-6(IL-6) secreted by ASMCs were detected by enzyme linked immunosorbent assay (ELISA). Result:Compared with blank group and normal rat serum group, the fluorescence intensity and protein expression of α-actin in model group and normal rat serum cell model group were low, whereas the fluorescence intensity and protein expression of OPN were high, and the concentrations of VEGF, TGF-β and IL-6 increased significantly (P<0.05). Compared with model group and normal rat serum cell model group, Saposhnikoviae Radix-Mume Fructus serum group and hormone intervention group could significantly enhance the fluorescence intensity and protein expression of alpha-actin, decrease the fluorescence intensity, protein expression of OPN and concentrations of VEGF, TGF-β and IL-6 (P<0.05). Conclusion:The combined administration of "Saposhnikoviae Radix-Mume Fructus" has an inhibitory effect on airway remodeling, which may be related to the inhibition of the transformation of ASMCs from contractile phenotype to synthetic phenotype, so as to reduce the release of active substances, such as VEGF, TGF-β and IL-6.
