Effect of Huangqi Guizhi Wuwu Tang on Angiogenesis in Osteoarthritis
10.13422/j.cnki.syfjx.20190338
- VernacularTitle: 黄芪桂枝五物汤对骨关节炎大鼠血管新生的作用
- Author:
Le ZHAO
1
;
Yan-yan LI
2
;
Yong-hui WANG
2
;
Xiao-jun DONG
3
;
Ran ZHOU
2
;
Ruo-nan ZHANG
2
;
Li-wei SUN
2
Author Information
1. Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
2. Shanxi University of Chinese Medicine, Taiyuan 030024, China
3. Zunyi Medical and Pharmaceutical College, Zunyi 563006, China
- Publication Type:Research Article
- Keywords:
Huangqi Guizhi Wuwu Tang;
osteoarthritis;
vascular endothelial growth factor (VEGF);
angiogenesis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(3):87-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of Huangqi Guizhi Wuwu Tang on angiogenesis of osteoarthritis with Yang deficiency and cold coagulation.Method: Totally 60 female SD rats were randomly divided into control group, model group, celecoxib group (20.82 mg·kg-1) and low, medium, high-dose Huangqi Guizhi Wuwu Tang groups (3.24, 6.48, 12.96 g·kg-1). All groups, except for control group, were involved in duplicating the osteoarthritis(OA) model through frozen and knee fixation, as well as 42-day cold environmental stimulation. After modeling, all drug-group rats were respectively administrated with corresponding drugs for 28 days, once a day. Meanwhile, control group and model group were given equivalent distilled water by gavage. 24 hours after the last gavage, vascular endothelial growth factor (VEGF),prostaglandin E2(PGE2) and transforming growth factor-β1(TGF-β1) in serum were detected with enzyme linked immunosorbent assay(ELISA) method, VEGF expressions in cartilage and synovial with immunohistochemical method, and interleukin-17(IL-17) and VEGF levels in synovial with Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR).Result: Compared with normal group, the expression of VEGF in serum, cartilage and synovial were significantly increased (P<0.01), and PGE2 and TGF-β1 expressions, IL-17 level were increased significantly (P<0.01) as well. Compared with model group, VEGF in serum, cartilage and synovial were significantly decreased in the 4 drug groups. Meanwhile, PGE2, TGF-β1 and IL-17 level were decreased significantly (P<0.05). Among drug groups, IL-17 of high-dose group in synovial was higher than that of celecoxib group (P<0.05), and VEGF of medium and high-dose Huangqi Guizhi Wuwu Tang groups in synovial was higher than that of celecoxib group (P<0.05). There was no significant difference among other drug groups.Conclusion: Huangqi Guizhi Wuwu Tang has an effect in suppressing angiogenesis of knee and alleviate cartilage lesion by regulating VEGF and its upstream cytokines PGE2 and TGF-β1.
