Effect of Leonurine on p38 MAPK Signaling Pathway and miRNA-1 in Cardiomyocyte Hypertrophy Induced by AngⅡ
10.13422/j.cnki.syfjx.20190337
- VernacularTitle: 益母草碱对血管紧张素Ⅱ诱导的心肌细胞肥大p38 MAPK信号通路和miRNA-1表达的影响
- Author:
Li-na LU
1
;
Zhao-wen LIANG
2
;
Jian-hua LUO
1
;
Li LI
1
;
Lu YUAN
1
;
Hui YANG
1
;
Dong-hua YANG
1
Author Information
1. The People's Hospital of Guizhou Province, Guiyang 550002, China
2. The Second Affiliated Hospital of Guiyang University of Traditional Chinese Medicine, Guiyang 550003, China
- Publication Type:Research Article
- Keywords:
leonurine;
cardiomyocyte hypertrophy;
p38 mitogen-activated protein kinase (p38 MAPK);
microRNA-1(miRNA-1)
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(3):81-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the inhibitory effect of leonurine on cardiomyocyte hypertrophy induced by angiotensin Ⅱ(Ang Ⅱ) and its effect on p38 mitogen-activated protein kinase (p38 MAPK) signaling pathway and miRNA-1.Method: Cardiomyocyte hypertrophy was induced by Ang Ⅱ (0.1 μmol·L-1) in primary neonatal cardiomyocytes. Experiments were designed in 6 groups as following:normal group, model group, p38 MAPK inhibitor group (SB203580, 10 μmol·L-1), low-dose(5 μmol·L-1), middle-dose(10 μmol·L-1) and high-dose(20 μmol·L-1) group. The cardiomyocyte surface area was measured by image software, and the protein contents were detected by Lowry. The concentrations of ANP in the culture supernatant were measured by enzyme-linked immunosorbent assay (ELISA). The expression level of miRNA-1 was detected by Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR), and the protein expression levels of endothelin-1 (ET-1), p38 MAPK, p-p38 MAPK, myocyte enhancer factor 2 (MEF2), β-myosin heavy chain (β-MHC), α-myosin heavy chain (α-MHC) were detected by Western blot.Result: Compared with normal group, the surface area of cardiomyocyte, the protein contents, the concentrations of ANP, and the protein expression levels of ET-1, p38 MAPK, p-p38 MAPK, MEF2, β-MHC in model group were higher (P<0.05), but the protein expression levels of α-MHC and miRNA-1 were lower than those in normal group (P<0.05). Compared with model group, the surface area of cardiomyocyte, the protein contents, the concentrations of ANP, and the protein expression levels of ET-1, p38 MAPK, p-p38 MAPK, MEF2, β-MHC in high-dose group were lower (P<0.05), but the protein expression levels of α-MHC and miRNA-1 were higher than those in model group (P<0.05).Conclusion: Leonurine (20 μmol·L-1) could inhibit cardiomyocyte hypertrophy induced by AngⅡ, and the mechanism is related to the inhibition of activation of p38 MAPK signaling pathway and up-regulation the expression of miRNA-1.
