Effect of Cooling Blood Method in Inhibiting Macrophage Apoptosis Against Atherosclerosis
10.13422/j.cnki.syfjx.20190336
- VernacularTitle: 凉血散瘀法通过抑制巨噬细胞凋亡抗动脉粥样硬化的作用
- Author:
Xue-qian LIU
1
;
Jing WANG
1
;
Shou-pei CAO
2
;
Yao-hong SONG
2
Author Information
1. Nanjing University of Chinese Medicine, Nanjing 210023, China
2. Nanjing Chinese Medicine Hospital Affilicated to Nanjing University of Chinese Medicine, Nanjing 210001, China
- Publication Type:Research Article
- Keywords:
cool blood method;
Qingxin Tongmai decoction;
atherosclerosis;
macrophage;
apoptosis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2019;25(3):59-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of serum-containing Qingxin Tongmai decoction(QXTMD) on the apoptosis rate of mouse mononuclear macrophage cell line RAW264.7 induced by Acetylated low density lipoprotein (ac-LDL) and the expressions of type A scavenger receptor(SR-A), B-cell lymphoma-2(Bcl-2), Bcl-2-associated X protein(Bax), inositol-requiring enzyme 1α(IRE1α), exploring the possible mechanism of QXTMD in the treatment of atherosclerosis.Method: Eight New Zealand rabbits were randomly divided into the atorvastatin group (2.6 g·kg-1) low, medium and high-dose QXTMD groups (3.33, 6.66, 13.32 mg·kg-1). After 7 days of gavage, the carotid blood was collected to prepare drug-containing serum. The RAW264.7 cell line was stimulated with 2.5%, 5%, 10%, and 20% drug-containing serum culture for 6, 12, and 24 h, respectively. The cell proliferation rate was observed by cell counting kit-8 (CCK-8) method. The RAW264.7 cell line was cultured in vitro and divided into blank group, model group, atorvastatin group, and low, medium and high-dose QXTMY groups. The cells in blank group were cultured with bovine serum albumin(BSA). The model group was stimulated with BSA+50 mg·L-1 ac-LDL for 24 h. The other groups were stimulated with BSA+50 mg·L-1 ac-LDL+10% drug-containing serum for 24 h. The apoptosis rate and SR-A expression of RAW264.7 cells were detected by flow cytometry. The expressions of Bcl-2, Bax and IRE1α protein were detected by Western blot.Result: Compared with the blank group, the model group could increase the apoptosis rate of RAW264.7 cells (P<0.01) and the expressions of Bax and SR-A protein (P<0.01), but decrease the expression of Bcl-2 protein (P<0.05). Compared with the model group, low, medium and high-dose QXTMD groups could decrease the apoptosis rate of RAW264.7 cell line (P<0.05) and the expressions of SR-A and IRE1α (P<0.05,P<0.01). The low-dose QXTMD group and the high-dose QXTMD group could decrease the expression of Bax(P<0.05,P<0.01). The middle-dose group and the high-dose group could decrease the expression of Bcl-2(P<0.01).Conclusion: QXTMD can reduce the apoptosis rate of macrophages. The mechanism of atherosclerosis may be related to the expressions of Bax, IREα, SR-A and anti-apoptotic protein Bcl-2.
