MTA2 highly expressed in bladder cancer tissues promotes the malignant biological behaviors of bladder cancerT24 cells
10.3872/j.issn.1007-385x.2019.06.009
- VernacularTitle:膀胱癌组织中高表达的MTA2促进膀胱癌细胞T24的恶性生物学行为
- Author:
PENG Kenan
1
,
2
,
3
;
LI Xiaoya
4
;
BAI Hanyu
4
;
WANG Gang
5
,
6
;
DAI Suli
4
;
YANG Tao
5
,
6
;
LIU Yujing
5
,
6
;
TAN He
5
,
6
;
ZHAO Ming
5
,
6
;
ZHAO Lianmei
4
;
Shan Baoen
4
Author Information
1. 1. Laboratory Department, People'
2. s Hospital of Hebei Province, Shijiazhuang 050011, Hebei, China
3. 2. Research Center, the Fourth Hospital of Hebei Medical University
4. Research Center, the Fourth Hospital of Hebei Medical University
5. Laboratory Department, People'
6. s Hospital of Hebei Province
- Publication Type:Journal Article
- Keywords:
metastasis-associated protein 2 (MTA2);
bladder cancer;
T24 cell line;
oncogene;
bio-marker
- From:
Chinese Journal of Cancer Biotherapy
2019;26(6):669-675
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of metastasis-associated protein 2 (MTA2) in human bladder cancer tissues and its effect on the malignant biological behaviors of bladder cancer T24 cells, as well as to explore the effect of MTA2 on the progression of bladder cancer. Methods: Sixty-two cases of human bladder cancer tissues and 28 cases of normal bladder tissues (from patients with cystitis, and pathologically confirmed as normal tissue) were collected at People’s Hospital of Hebei Province during December 2012 and December 2014. The expression of MTA2 in bladder cancer tissues and normal bladder tissues was detected by immunohistochemical staining, and the correlation between MTA2 expression and clinicopathological characteristics of patients was also analyzed. The bladder cancer T24 cell line stably expressing MTA2 was constructed. The effects of MTA2 on the proliferation, colony formation, migration and invasion of bladder cancer T24 cells were detected by MTS, clone formation, scratch healing and Transwell assay, respectively. Results: Immunohistochemical staining showed that MTA2 expression was significantly up-regulated in bladder cancer tissues as compared with normal bladder tissues (P<0.01). The high expression of MTA2 in bladder cancer tissues was not related to gender, age and tumor volume (P>0.05), but was associated with higher TNM stage, histological grade, and lymphatic infiltration and metastasis (all P<0.05). After over-expression of MTA2 in bladder cancer T24 cell line, the proliferation activity of the cells was significantly increased (P<0.05), and the colony formation, scratch healing, migration and invasion ability were significantly increased (all P<0.01). Conclusions: MTA2 is up-regulated in human bladder cancer tissues and can promote the proliferation, tumor formation, migration and invasion of T24 cells.
- Full text:20190609.pdf
