AKT/MMP3 activation, not EMT pathway, participates in CCL20-induced invasion and migration of colon cancer SW480 cells
DOI ： ： 10.3872/j.issn.1007-385x.2019.06.006
- VernacularTitle:CCL20通过AKT/MMP3轴而非EMT途径诱导结肠癌SW480细胞的侵 袭和转移
- Author:
CHENG Xianshuo
1
;
YANG Fang
1
;
DONG Jian
1
;
LI Yunfeng
1
;
YANG Zhibin
2
;
ZHANG Hongtao
1
;
SHEN Tao
1
;
LIU Ping
1
;
YIN Zhengfeng
1
;
LI Qiang
1
Author Information
1. Colorectal Surgery, the Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province
2. Molecular Oncology Laboratory, Eastern Hepatobiliary Surgery Hospital
- Publication Type:Journal Article
- Keywords:
colorectal cancer;
CCL20;
CCR6;
matrix metalloproteinase 3 (MMP3);
epithelial-mesenchymal transition (EMT)
- From:
Chinese Journal of Cancer Biotherapy
2019;26(6):650-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the molecular mechanism of chemokine CCL20/CCR6 in promoting invasion and migration of colon cancer SW480 cells. Methods: Colorectal cancer SW480 cells with high expression of CCR6 receptor were screened by immunochemistry (IHC). After co-culture with recombinant human CCL20, the invasion and migration of SW480 cells were detected by Transwell assay and Wound-Healing assay, respectively. Expressions of EMT markers, AKT signal protein and target protein MMP3 were detected by immunofluorescence (IF) and WB. AKT signaling pathway as the key mechanism was confirmed by MK2206 blocking assay. The expressions of CCL20 and MMP3 in colorectal cancer tissues as well as their correlation were analyzed by TCGAdatabase resources (https://portal.gdc.cancer.gov/). Results: CCL20 promoted the invasion and migration ability of SW480 cells significantly (all P <0.01), and this was induced by activation of AKT signaling and up-regulation of downstream target protein MMP3, instead of EMT. Blocking AKT signaling could significantly inhibit the invasion and migration of SW480 cells, and down-regulate MMP3 expression (P<0.05). TCGA platform data showed that the expressions of CCL20 and MMP3 in colorectal cancer tissues were significantly higher than those in normal mucosa tissues (P<0.05 or P<0.01), and an evidently positive correlation was found between CCL20 and MMP3 (r =0.051, P<0.01). Conclusion: The chemokine CCL20 promotes the invasion and migration of SW480 cells throughAKT/MMP3 signal axis, but not the EMT.
- Full text:20190606.pdf
