Effect of TET1-CD on proliferation and migration of breast cancer MDA-MB-231 cells and its underlying mechanism
10.3872/j.issn.1007-385x.2019.06.005
- VernacularTitle:TET1-CD对乳腺癌细胞MDA-MB-231增殖和迁移的影响及其作用机制
- Author:
ZHAO Quanhua
1
;
WANG Shensen
1
;
MA Ling
1
;
ZHOU Zhixiang
1
;
HUANG Yinghui
1
Author Information
1. (College of Life Sciences and Bioengineering, Beijing University of Technology
- Publication Type:Journal Article
- Keywords:
breast cancer;
MDA-MB-231 cell;
TET1 catalytic domain (TET1-CD);
epigenetic;
proliferation;
migration;
epithelial mesenchymal transformation (EMT)
- From:
Chinese Journal of Cancer Biotherapy
2019;26(6):644-649
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of high expression of TET1 catalytic domain (TET1-CD) gene on the proliferation and migration of breast cancer MDA-MB-231 cells and its underlying mechanism. Methods: MDA-MB-231 cell line with high TET1-CD expression was established by lentiviral transfection. Real-time quantitative PCR was used to detect the mRNA expression of TET1-CD. Transwell assay and cell scratch assay were used to detect cell migration ability, MTT assay and colony formation assay were used to detect cell proliferation capacity. And WB was adopted to detect the expressions of EMT-related proteins (E-cadherin, Vimentin, MMP2) and Wnt, Hedgehog pathway-related proteins in MDA-MB-231 cells. Results: The MDA-MB-231 cell line with high TET1-CD expression was successfully constructed (all P<0.01). TET1-CD over-expression significantly inhibited the proliferation and migration of breast cancer MDA-MB-231 cells (P<0.01); in addition, TET1-CD over-expression increased the expression of E-cadherin, but down-regulated the expressions of Vimentin, MMP2, β-catenin, Gli1, C-myc and CyclinD1 (all P<0.05). Conclusion: TET1-CD may inhibit the proliferation and migration of breast cancer MDA-MB-231 cells by inhibiting the EMT through Wnt and HH signaling pathway.
- Full text:20190605.pdf
