Determination of nine effective constituents in Chenxiang-Huaqi tablets by quantitative analysis of multi-components by single marker
10.3760/cma.j.issn.1673-4246.2019.09.016
- VernacularTitle: 一测多评法测定沉香化气片中9种活性成分的含量
- Author:
Wencai WANG
1
;
Lei JIN
2
;
Guiping ZHANG
2
Author Information
1. Pharmaceutical Clinical Trial Research Section of Qingdao Municipal Hospital, Qingdao 266000, China
2. Central Pharmacy of Pharmacy Department of Qingdao Municipal Hospital, Qingdao 266000, China
- Publication Type:Journal Article
- Keywords:
Assay (TCD);
Chenxiang-Huaqi tablets;
Quantitative analysis of multi-components by single marker;
Liquiritin;
Costunolide;
Dehydrocostus lactone;
Narirutin;
Naringin;
Hesperidin;
Neohesperidin;
Pogostone;
High performance liquid chromatography
- From:
International Journal of Traditional Chinese Medicine
2019;41(9):986-991
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a method of quantitative analysis of multi-components by single marker for nine effective constituents in Chenxiang-Huaqi tablets.
Methods:Using HPLC method, the mobile phase was acetonitrile -0.2% physcion acid in gradient elution program, the flow rate was 1.0 ml/min. The detection wavelength was set at 230 nm for liquiritin, ammonium glycyrrhizinate, costunolide and dehydrocostus lactone, and 283 nm for narirutin, naringin, hesperidin and neohesperidin, and 310 nm for pogostone. The column temperature was 30 ℃ and the injection volume was 10 μl. Using Hesperidin as the internal standard, the relative correction factors among liquiritin, ammonium glycyrrhizinate, costunolide, dehydrocostus lactone, narirutin, naringin, neohesperidin, pogostone were detected by QAMS and their contents were calculated. The content difference of the above nine components with the external standard method were compared.
Results:The calculated values of hesperidin, liquiritin, ammonium glycyrrhizinate, costunolide, dehydrocostus lactone, narirutin, naringin, neohesperidin, pogostone were not statistical significance from those measured by external standard method (P>0.05).
Conclusions:The method offered reference for quality standard of Chenxiang-Huaqi tablets.
