Application value of interferon gamma release assay combined with tuberculin skin test in diagnosis of intraocular tuberculosis and latent tuberculosis infection
10.3760/cma.j.issn.1673-4904.2019.09.006
- VernacularTitle: 干扰素γ释放试验联合结核菌素皮肤试验在眼内结核和潜伏性结核感染诊断中的应用价值
- Author:
Yankun ZHANG
1
;
Hongzhong BAI
2
;
Yan GUAN
1
;
Yujing LI
3
;
Xiaoying AN
4
;
Chao HAN
1
Author Information
1. Department of Ophthalmology, Hebei Chest Hospital, Shijiazhuang 050041, China
2. Department of Radiology, Hebei Chest Hospital, Shijiazhuang 050041, China
3. Department of Laboratory, Hebei Chest Hospital, Shijiazhuang 050041, China
4. Molecular Biology Laboratory, Hebei Chest Hospital, Shijiazhuang 050041, China
- Publication Type:Journal Article
- Keywords:
Tuberculosis, ocular;
Tuberculin test;
Latent tuberculosis;
Interferon gamma release assay
- From:
Chinese Journal of Postgraduates of Medicine
2019;42(9):791-795
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the application value of interferon gamma release assay (IGRA) combined with tuberculin skin test (TST) in diagnosis of intraocular tuberculosis and latent tuberculosis infection.
Methods:One hundred and ten patients with pulmonary tuberculosis (observation group) and 80 healthy persons (control group) in Hebei Chest Hospital from January 2016 to December 2018 were selected. Among the 110 patients with pulmonary tuberculosis, intraocular tuberculosis was in 35 cases, and latent tuberculosis infection was in 75 cases. All the patients were examined by ocular secretions IGRA and TST.
Results:The positive rates of IGRA and TST in observation group were significantly higher than those in control group: 88.18% (97/110) vs. 11.25% (9/80) and 83.64% (92/110) vs. 41.25% (33/80), and there were statistical differences (χ2 = 113.138 and 36.971, P<0.01). The positive rate of IGRA in patients with intraocular tuberculosis was significantly higher than that in latent tuberculosis infection: 97.14% (34/35) vs. 84.00% (63/75), and there was statistical difference (χ2 = 3.955, P<0.05). There was no statistical difference in the positive rate of TST between the patients with intraocular tuberculosis and patients with latent tuberculosis infection: 91.43% (32/35) vs. 80.00% (60/75), χ2 = 2.277, P>0.05. The consistency rate of IGRA and TST was 89.90% (Kappa = 0.867, P>0.05). The sensitivity of IGRA combined with TST was significantly higher than the single detection of IGRA and TST (97.83% vs. 88.18% and 83.64%), while the specificity of IGRA combined with TST was significantly lower than the single detection of IGRA and TST (61.11% vs. 92.31% and 83.33%), and there were statistical differences (P<0.05). The receiver operating characteristic curve analysis result showed that the area under curve of IGRA in diagnosis of intraocular tuberculosis and latent tuberculosis infection was 0.781, area under curve of TST was 0.604, and area under curve of IGRA combined with TST was 0.679.
Conclusions:IGRA has high value in the diagnosis of intraocular tuberculosis and latent tuberculosis infection, and can be used as a diagnostic means of intraocular tuberculosis.
