Effect of minimally invasive drilling closed drainage on the expression of HIF-1α, Claudin-5, TGF- β and Smad2/3 in patients with chronic subdural hematoma
10.3760/cma.j.issn.1008-1372.2019.09.014
- VernacularTitle: 微创钻孔密闭引流术对慢性硬膜下血肿患者HIF-1α、Claudin-5、TGF-β及Smad2/3表达的影响
- Author:
Xuejun GAO
1
;
Jingbo ZENG
;
Qiang ZHAO
;
Dongbin MA
Author Information
1. Department of Neurosurgery, the fifth People′s Hospital of Chengdu, Chengdu 611130, China
- Publication Type:Journal Article
- Keywords:
Drainage;
Hematoma, subdural, chronic;
Hypoxia-inducible factor 1, alpha subunit;
Claudin-5;
Transforming growth factor beta;
Smad proteins, receptor-regulated
- From:
Journal of Chinese Physician
2019;21(9):1345-1348
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of single-hole and double-hole drilling and closed drainage on the expression of Hypoxia inducible factor-1α (HIF-1α), Claudin-5, transforming growth factor-β (TGF-β) and Smad2/3 in the epidermis of patients with chronic subdural hematoma.
Methods:100 patients with chronic subdural hematoma were randomly divided into single hole and double hole drainage group according to random number table. Immunohistochemical streptavidin-peroxidase (SP) was used to detect the expression of TGF- β protein, and Western blot was used to detect the expression of HIF-1α, Claudin-5 and Smad2/3 in the epihematoma of the two groups before and after operation.
Results:Compared with pre-operation, the expression of HIF-1α, TGF-β and Smad2/3 protein in adventitia of hematoma in both groups decreased after operation, especially in the double-hole group (P<0.05). The expression of Claudin-5 protein in adventitia of hematoma was significantly increased in both groups, especially in the double-hole group, with statistically significant difference (P<0.05).
Conclusions:Single-hole and double-hole drilling and sealing drainage is an effective method to treat chronic subdural hematoma. It can reduce the expression of HIF-1α, TGF-β and Smad2/3 protein in the epidermis of hematoma, and significantly increase the expression of Claudin-5, and the effect of double-hole drilling and sealing drainage is more significant.
