Controlled delivery of glial cell line-derived neurotrophic factor enhances motor nerve regeneration
10.3760/cma.j.issn.1008-1372.2019.09.009
- VernacularTitle: 胶质细胞源性神经营养因子缓释给药对股神经运动神经支损伤修复效果的实验研究
- Author:
Zhihui WANG
1
;
Xiaokun GENG
2
Author Information
1. Changsha Health Vocational College, Changsha 410100, China
2. Department of Neurology, Beijing Luhe Hospital Affiliated to Capital Medical University, Beijing 101149, China
- Publication Type:Journal Article
- Keywords:
Glial cell line-derived neurotrophic factor;
Medication systems;
Femoral neuropathy;
Nerve regeneration;
Rats
- From:
Journal of Chinese Physician
2019;21(9):1321-1325
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine the effect of a motor-specific neurotrophic factor, glial-derived neurotrophic factor (GDNF) on motor nerve regeneration.
Methods:We used a nerve conduit filled with a fibrin-based delivery system that provided controlled release of GDNF during nerve regeneration. The motor branch of the rat femoral nerve was used to assess motor nerve regeneration across a 5-mm gap. Four experimental groups (n=5) were evaluated. These included GDNF with the fibrin-based delivery system (GDNF-DS group), fibrin alone(fibrin group), empty conduit (negative control group), and nerve isograft (positive control group). Nerves were harvested at 5 weeks for analysis by histomorphometry and electron microscopy.
Results:At 5 mm distal to the conduit or isografts, the GDNF-DS group was not significantly different from the nerve isograft group in the following histomorphometric measures: total nerve fibers, percentage of neural tissue, and nerve density. The number of nerve fibers (respectively: 1 744±274 , 1 481±288) and the percentage of nerve tissue [(14.2±3.9)%, (11.0±2.2)%] in theisograft group and the GDNF-DS group were significantly higher than that in the fibrin group and the empty conduit group [(respectively: 538±93, 535±96) and the percentage of nerve tissue respectively: (4.3±1.6)%, (3.7±0.9)%]. There were no differences in fiber width among all groups. By electron microscopy, the GDNF-DS and isograft groups also demonstrated more organized nerve architecture than the fibrin alone and empty conduit groups.
Conclusions:The delivery of GDNF from the fibrin-based delivery system promotes motor nerve regeneration at a level similar to an isograft in the femoral motor nerve model. This study gives insight into the potential beneficial role of GDNF in the treatment of motor nerve injuries.
