Role of microRNA-125b in ventilator-induced lung injury in mice
10.3760/cma.j.issn.0254-1416.2019.09.024
- VernacularTitle: 微小RNA-125b在小鼠呼吸机相关性肺损伤中的作用
- Author:
Tianfeng HUANG
1
;
Ju GAO
;
Luojing ZHOU
;
Yali GE
;
Cunjin WANG
Author Information
1. Department of Anesthesiology, Institute of Anesthesiology and Critical Care, Northern Jiangsu People′s Hospital Affiliated to Yangzhou University, Yangzhou 225001, China
- Publication Type:Journal Article
- Keywords:
MicroRNAs;
Ventilator-induced lung injury
- From:
Chinese Journal of Anesthesiology
2019;39(9):1121-1124
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the role of microRNA-125b (miR-125b) on ventilator-induced lung injury (VILI) in mice.
Methods:Forty healthy male C57BL/6 male mice, weighing 25-30 g, aged 2-3 months, were divided into 4 groups (n=10 each) using a random number table method: sham operation group (group Sham), group VILI, VILI plus miR-125b negative control group (group VILI+ NC), and VILI plus miR-125b overexpression group (group VILI+ miR-125b agomir). In VILI+ NC and VILI+ miR-125b agomir groups, miR-125b negative control and miR-125b agomir transfection complex 50 μl were intratracheally instilled, respectively, and 48 h later VILI model was established.The animals were mechanically ventilated for 4 h with high tidal volume (40 ml/kg) to induce VILI.Blood samples were obtained from the femoral artery at 4 h of mechanical ventilation for detection of PaO2, then animals were sacrificed, lungs were removed for determination of wet to dry weight ratio (W/D ratio) and for examination of pathological changes (with a light microscope), and lung injury was scored.In VILI+ NC and VILI+ miR-125b agomir groups, bronchoalveolar lavage fluid (BALF) was collected to measure the concentrations of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) by enzyme-linked immunosorbent assay.The cell apoptosis of lung tissues was measured using TUNEL, apoptosis index was calculated, the caspase-3 expression was detected by Western blot, and the miR-125b expression was detected by real-time polymerase chain reaction.
Results:Compared with Sham group, PaO2 was significantly decreased, and W/D ratio and lung injury score were increased in VILI, VILI+ NC and VILI+ miR-125b agomir groups, and the expression of miR-125b was down-regulated in VILI and VILI+ NC groups (P<0.05). Compared with VILI group, PaO2 was significantly increased, W/D ratio and lung injury score were decreased, the expression of miR-125b was up-regulated (P<0.05), the pathological changes of lung tissues were significantly attenuated in group VILI+ miR-125b agomir, and no significant change was found in the parameters mentioned above in group VILI+ NC (P<0.05). Compared with group VILI+ NC, the concentrations of TNF-α and IL-6 in BALF and apoptotic index were significantly decreased, and the expression of caspase-3 was down regulated in group VILI+ miR-125b agomir (P<0.05).
Conclusion:MiR-125b is involved in the endogenous protective mechanism of VILI in mice.
