Role of spinal COX-1 and COX-2 in remifentanil-induced hyperalgesia in mice with incisional pain
10.3760/cma.j.issn.0254-1416.2019.09.016
- VernacularTitle: 脊髓COX-1和COX-2在瑞芬太尼诱发切口痛小鼠痛觉过敏形成中的作用
- Author:
Zhongfei WANG
1
;
Zhen WANG
;
Linlin ZHANG
;
Yize LI
;
Yuzhu TAO
;
Zicheng WANG
;
Keliang XIE
;
Yonghao YU
;
Guolin WANG
Author Information
1. Department of Anesthesiology, Tianjin Medical University General Hospital Tianjin Research Institute of Anesthesiology, Tianjin 300052, China
- Publication Type:Journal Article
- Keywords:
Prostaglandin-endoperoxide synthases;
Piperidines;
Pain, postoperative;
Hyperalgesia
- From:
Chinese Journal of Anesthesiology
2019;39(9):1088-1091
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the role of spinal COX-1 and COX-2 in remifentanil-induced hyperalgesia in mice with incisional pain.
Methods:Thirty-two male C57BL/6J mice, aged 8-10 weeks, weighing 20-25 g, were divided into 4 groups (n=8 each) using a random number table method: control group (group C), incisional pain plus remifentanil group (group IR), incisional pain plus remifentanil plus selective COX-1 inhibitor group (group IR+ SC560), and incisional pain plus remifentanil plus selective COX-2 inhibitor group (group IR+ SC236). In IR, IR+ SC560 and IR+ SC236 groups, normal saline 10 μl, SC560 25 μg and SC236 25 μg were intrathecally injected, respectively, 15 min later remifentanil 10 μg/kg was injected via the tail vein for 4 times at 15 min intervals.An incisional pain model was established after the first injection of remifentanil.The mechanical paw withdrawal threshold (MWT) was measured at 24 h before normal saline or remifentanil injection and 3, 6, 24 and 48 h after the last injection (T0-T4). The mice were sacrificed after the last measurement of pain threshold, and the L4-6 segments of the spinal cord were removed for determination of the expression of COX-1 and COX-2 (by Western blot) and expression of COX-1 and COX-2 mRNA (by quantitative real-time polymerase chain reaction).
Results:Compared with group C, the MWT was significantly decreased, and the expression of COX-2 protein and mRNA was up-regulated in IR, IR+ SC560 and IR+ SC236 groups (P<0.05). Compared with group IR, the MWT was significantly increased in IR+ SC560 and IR+ SC236 groups (P<0.05). There was no significant difference in the MWT at each time point between IR+ SC560 and IR+ SC236 (P>0.05) .There was no significant difference in the expression of COX-2 protein and mRNA among group IR, group IR+ SC560 and group IR+ SC236 (P>0.05). There was no significant difference in the expression of COX-1 protein and mRNA among the four groups (P>0.05).
Conclusion:Compared with COX-1, spinal COX-2 plays a major role in the pathophysiological mechanism of remifentanil-induced hyperalgesia in mice with incisional pain.
