Analysis on the hemagglutinin genetic characteristic of 7 measles virus isolated in Henan Province in 2017
10.3760/cma.j.issn.0253-9624.2019.09.012
- VernacularTitle: 2017年河南省7株麻疹病毒分离株血凝素基因特征分析
- Author:
Daxing FENG
1
;
Lu ZHANG
;
Wanyu LYU
;
Jianhui YANG
;
Guangwei LI
;
Jin XU
;
Yanyang ZHANG
Author Information
1. Institute of Expanded Programme on Immunization, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016, China
- Publication Type:Journal Article
- Keywords:
Measles virus;
Hemagglutinin;
Characterization;
Surveillance
- From:
Chinese Journal of Preventive Medicine
2019;53(9):929-933
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Analyze the genetic characteristic of Hemagglutinin(H) gene of measles viruses isolated in Henan Province in 2017.
Methods:Swab samples collected from 7 lab confirmed measles cases, and we got the measles virus by Vero/Slam inoculation. Fragment of H genes were amplified by reverse transcription polymerase chain reaction(RT-PCR), then the PCR products were sequenced and analyzed.
Results:The age of the 7 measles confirmed cases were between 1 and 50 years old, and all of them were males. All the 7 measles viruses were identified as H1a genotype, and the average distance of the nucleotides and the amino acids was 0.005, respectively. Compared with the Shanghai-191/China-vaccine, there were some changes in isolated virus, such as 240th, 397th and 381st sites in the amino acid sequence.
Conclusion:The measles genotype which isolated in Henan Province in 2017 was H1a. There were some difference from Shanghai-191/China-vaccine in the nucleotides sequence of H gene, which suggested that it′s necessary to strengthen the monitor the variation of measles virus.
