Peritoneal dialysis improves lung function in rats with lung injury induced by shock wave
10.3760/cma.j.issn.1001-7097.2019.09.007
- VernacularTitle: 腹膜透析改善大鼠冲击伤后肺功能
- Author:
Wei HU
1
,
2
;
Kehong CHEN
1
;
Xiaoyue WANG
1
;
Juan DU
3
;
Yani HE
1
Author Information
1. Department of Nephrology, Army Characteristic Medical Center, Chongqing 400010, China
2. Department of Nephrology, Army 958 Hospital, Chongqing 400020, China
3. Department of NO.4 Field Surgery, Army Special Medicine Center, Chongqing 400010, China
- Publication Type:Journal Article
- Keywords:
Peritoneal dialysis;
Lung injury;
Cytokines;
Inflammation;
Respiratory function tests
- From:
Chinese Journal of Nephrology
2019;35(9):684-689
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prove the efficacy of peritoneal dialysis on shock wave-induced acute lung injury of rats, and analyze its mechanisms.
Methods:Forty-five adult Sprague-Dawley rats were randomly divided into three groups: control group, sham operation (Sham) group and peritoneal dialysis (PD) group. Sham group and PD group did abdominal catheterization before blast injury. The 55 kg shock wave (bst-I) was used to induce lung blast injury. After one hour of blast injury, PD group was given 2.5% peritoneal dialysate 20 ml to stay abdomen, which was released 30 min posted, repeated 12 cycles. After 6 hours of peritoneal dialysis, all of the rats were sacrificed. Partial damaged tissues in lung were used to evaluate the pathomorphologic changes by HE staining, and the remnants were used to measure the lung water content. Lung function was detected by blood gas analyzer and small animal detector from the arterial blood gas. The levels of serum inflammatory factors, such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 and monocyte chemoattractant protein-1 (MCP-1) were tested by ELISA.
Results:The relative integrity of alveolar structure, interstitial edema and inflammatory cell infiltration in PD group were significantly improved than those in control group. The lung water content of PD group was significantly lower than that of control group (P<0.05). The levels of TNF-α, IL-1β, IL-6 and MCP-1 in serum of PD group were significantly lower than those in control group (all P<0.05). The blood oxygen saturation, oxygen partial pressure, oxygenation index, vital capacity, functional residual volume and maximum mid-expiratory flow rate in PD group were significantly higher than those in control group (all P<0.05).
Conclusions:Through reducing pulmonary edema and inflammatory factors, peritoneal dialysis can improve lung function in shock wave -induced acute lung injury of rats.
