Multi locus sequence typing and antibiotic susceptibility of extended-spectrum beta-lactamases producing <italic>Enterobacteriaceae</italic> in rural residents in villages with pig-breeding farms in Shandong province

Aiying Teng; Liuchen XU; Peng Yang; Chengyun Sun; Baoli Chen; Shuang Wang; Zengqiang Kou; Ming Fang; Miaomiao Wang; Zhenqiang BI

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Multi locus sequence typing and antibiotic susceptibility of extended-spectrum beta-lactamases producing Enterobacteriaceae in rural residents in villages with pig-breeding farms in Shandong province

VernacularTitle: 山东省某地产超广谱β-内酰胺酶大肠埃希菌耐药情况及多位点序列分型
Author: Aiying TENG ¹ ; Liuchen XU ² ; Peng YANG ³ ; Chengyun SUN ³ ; Baoli CHEN ² ; Shuang WANG ² ; Zengqiang KOU ² ; Ming FANG ² ; Miaomiao WANG ⁴ ; Zhenqiang BI ^{1
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Author Information

1. Department of Epidemiology, School of Public Health, Shandong University, Jinan 250012, China
2. Institute for Bacterial Infectious Disease Control and Prevention, Shandong Provincial Center for Disease Control and Prevention, Jinan 250014, China
3. Zhucheng County Center for Disease Control and Prevention, Zhucheng 262200, China
4. Affiliated Hospital of Jining Medical University, Jining 272029, China
5. Shandong Provincial Center for Disease Control and Prevention, Jinan 250014, China
6. Shandong Key Laboratory for Communicable Disease Control and Prevention, Jinan 250014, China
7. Shandong University Institute of Preventive Medicine, Jinan 250014, China
Publication Type:Journal Article
Keywords: Escherichia coli; Extended-spectrum β -lactamases; Multilocus sequence typing
From: Chinese Journal of Epidemiology 2019;40(9):1145-1149
CountryChina
Language:Chinese
Abstract: Objective:To analyze the antimicrobial resistance and multilocus sequence typing (MLST) results of extended-spectrum β-lactamase (ESBLs)-producing Escherichia coli in rural residents in villages with pig breeding farms in a county of Shandong province.
Methods:Antimicrobial susceptibility testing was performed with agar dilution method by using 360 ESBLs-producing E. coli strains from fresh stool samples of rural residents in villages with pig breeding farms in a county of Shandong. PCR was conducted to amplify the CTX-M, TEM, SHV genes and capillary electrophoresis was used to screen positive strains in July, 2016. MLST was performed for molecular typing analysis, and eBURST v3.0 software was used for cluster analysis.
Results:Among 360 strains of ESBLs-producing E. coli, the resistance rates to cefotaxime, tetracycline, trimethoprim-sulfamethoxazole and florfenicol were 100.0% (360/360), 82.2% (296/360), 81.1% (292/360) and 80.3% (289/360), respectively. The positive rate of CTX-M gene was 99.2% (357/360), in which the positive rate of CTX-M-9 was 35.6% (128/360) and the positive rate of CTX-M-1 was 24.4% (88/360). The positive rate of TEM gene was 26.9% (97/360). A total of 132 STs were identified through MLST. The predominant ST was ST10, accounting for 12.5% (45/360). Cluster analysis showed that CC10 was the most important clone group, including 39 ST clones, involving 148 strains (41.1%).
Conclusions:The drug resistances of ESBLs-producing E. coli to cefotaxime, tetracycline, trimethoprim-sulfamethoxazole and flurfenicol are serious in this rural area. There is a small-scale clustering of CC10 and transmission mode from animals to humans might exist.